公开(公告)号：US20230212593A1

公开(公告)日：2023-07-06

申请号：US17905499

申请日：2021-03-01

Applicant: BASF SE

Inventor： Max Fabian FELLE ,  Christopher SAUER ,  Norma WELSCH ,  Mathis APPELBAUM ,  Thomas SCHWEDER

IPC: C12N15/69 ,  C12N15/10 ,  C12N15/75

CPC classification number: C12N15/69 ,  C12N15/1034 ,  C12N15/75 ,  C12N2310/20 ,  C12N2820/55

Abstract: Disclosed herein are methods for the production of low to medium expressing constitutive promoters in bacteria and promoters produced therewith.

公开(公告)号：US20230139192A1

公开(公告)日：2023-05-04

申请号：US17905433

申请日：2021-03-01

Applicant: BASF SE

Inventor： Max Fabian FELLE ,  Christopher SAUER ,  Norma WELSCH ,  Mathis APPELBAUM ,  Thomas SCHWEDER

IPC: C12N15/75 ,  C12N15/70 ,  C12N15/63 ,  C12N9/22

Abstract: Disclosed herein is a shuttle vector for use in E. coli and Bacilli including a high copy replication origin functional in E. coli, a low to medium copy ORI functional in Bacilli, and a synthetic constitutive regulatory nucleic acid conferring reduced constitutive expression compared to a respective starting regulatory nucleic acid molecule in a bacterial cell.