公开(公告)号：US20210350877A1

公开(公告)日：2021-11-11

申请号：US17313707

申请日：2021-05-06

Applicant: BATTELLE MEMORIAL INSTITUTE

Inventor： Rachel R. Spurbeck ,  Christopher Johnson ,  Jacob Lilly ,  Jared M. Schuetter ,  Patrick H. Wilson

IPC: G16B50/00 ,  G16B25/00 ,  C12Q1/70 ,  B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  A61F13/38 ,  G01N33/18 ,  G01N21/78 ,  G16H10/40 ,  G16H50/20

Abstract: A point of care device, comprises a chamber configured to contain a swab. Once a swab is loaded, the device introduces a buffer into the chamber, then lyses cells collected by the swab. Probes such as padlock probes target a specified content such as a virus (e.g., SARS-CoV-2 RNA) and will bind, splint ligate, and rapidly isothermally amplify (e.g., using rolling circle amplification). Also described is a wastewater pathogen tracking dashboard and schema for detecting and tracking pathogen spread within communities by monitoring microbial and viral RNA.