公开(公告)号：US3891853A

公开(公告)日：1975-06-24

申请号：US45298074

申请日：1974-03-20

申请人： BAXTER LABORATORIES INC

发明人： KREMEN JEROME C ,  LANDA ISAAC

IPC分类号： G01J3/443 ,  G01J3/44 ,  G01N21/64 ,  G01N21/52

CPC分类号： G01J3/4406

摘要： An energy-compensated spectrofluorometer of the time-shared double beam type employing a rotating chopper mirror disc providing a main measure channel as one of the time-shared outputs of a photomultiplier tube and a compensating reference channel generated by a reference light whose output is conveyed by a light pipe to the cathode of the photomultiplier tube, generating the other time-shared output thereof. The energy correction is derived from a thermal detector cell receiving part of the excitation beam, and the reciprocal of the thermal detector cell signal is subtracted from the dark currentcorrected reference channel signal and is employed to adjust the high voltage supplied to the photomultiplier tube, thereby adjusting its gain. The measure channel includes means to compensate the measure signal for non-uniform spectral sensitivity of the photomultiplier tube, as well as for dark current. The reference channel signal is also modified to compensate for wavelength-dependent variations of quantum intensity in the excitation beam.

摘要翻译： 采用旋转斩光镜光盘的时分双光束型能量补偿分光荧光计，其提供作为光电倍增管的时间共享输出之一的主测量通道和由其输出的参考光产生的补偿参考通道 通过光管连接到光电倍增管的阴极，产生其它时间共享的输出。 能量校正是从接收部分激发光束的热检测器单元导出的，并且从暗电流校正参考通道信号中减去热检测器单元信号的倒数，并用于调节提供给光电倍增管的高电压 ，从而调整其增益。 测量通道包括用于补偿光电倍增管的非均匀光谱灵敏度的测量信号以及暗电流的装置。 参考通道信号也被修改以补偿激发光束中量子强度的波长相关变化。