公开(公告)号：US10400249B2

公开(公告)日：2019-09-03

申请号：US15124861

申请日：2015-03-09

Applicant: BAYER CROPSCIENCE AKTIENGESELLSCHAFT ,  BAYER CROPSCIENCE LP

Inventor： Fabien Poree ,  Bernd Laber ,  Gudrun Lange ,  Manuel Dubald ,  Roxanne Armstrong

IPC: C12N15/82 ,  A01N43/713 ,  C12N9/02

Abstract: In the present invention, HPPD enzymes and plants containing them showing a full tolerance against several classes of HPPD-inhibitors are described.A set of HPPD enzymes have been designed which have either no or only a significantly reduced affinity to HPPD inhibitors and, at the same time, the rate of dissociation of the HPPD inhibitors of the enzyme is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow, tight-binding inhibitors but, instead of this, have become fully reversible inhibitors.In particular, isolated polynucleotides encoding HPPD inhibitor tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

公开(公告)号：US10159249B2

公开(公告)日：2018-12-25

申请号：US15036690

申请日：2014-11-25

Applicant: BAYER CROPSCIENCE AKTIENGESELLSCHAFT

Inventor： Fabien Poree ,  Christian Waldraff ,  Bernd Laber ,  Arnim Koehn ,  Elmar Gatzweiler ,  Klaus Trabold

IPC: A01N43/713 ,  A01N43/68 ,  A01N43/707 ,  A01N57/20 ,  A01N25/00 ,  A01N43/70

Abstract: The use of 2-chloro-3-(methylsulfanyl)-N-(1-methyl-1H-tetrazol-5-yl)-4-(trifluoromethyl)benzamide or its salts for controlling unwanted plants in areas of transgenic crop plants being tolerant to HPPD inhibitor herbicides by containing one or more chimeric gene(s) comprising (I) a DNA sequence encoding hydroxyphenylpyruvate dioxygenase (HPPD) derived from a member of a group of organisms consisting of (a) Avena, (b) Pseudomonas, (c) Synechococcoideae, (d) Blepharismidae, (e) Rhodococcus, (f) Picrophilaceae, (g) Kordia, or (II) one or more mutated DNA sequences of HPPD encoding genes of the before defined organisms, preferably from Pseudomonas, or (III) one or more DNA sequences encoding mutated maize (Zea mays) or soybean (Glycine max) HPPD each being mutated as described in WO 2012/021785.