公开(公告)号：US20240200040A1

公开(公告)日：2024-06-20

申请号：US17787955

申请日：2019-12-23

申请人： BGI SHENZHEN

发明人： Qun AN ,  Miaomiao GUO ,  Feng XI ,  Fei GUO ,  Yue ZHENG ,  Yuliang DONG ,  Wenwei ZHANG

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/48

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/485 ,  C12Y207/07007

摘要： A chimeric DNA polymerase includes: a first fragment having at least 80% homology to at least part of an N-end domain of KOD DNA polymerase; a second fragment having at least 80% homology to at least part of an exonucleolytic domain of Pab DNA polymerase; a third fragment having at least 80% homology to at least part of the N-end domain of KOD DNA polymerase; a fourth fragment having at least 80% homology to at least part of a palm domain of Pfu DNA polymerase; a fifth fragment having at least 80% homology to at least part of a finger domain of Pab DNA polymerase; a sixth fragment having at least 80% homology to at least part of the palm domain of Pfu DNA polymerase; and a seventh fragment having at least 80% homology to at least part of a thumb domain of KOD DNA polymerase.