公开(公告)号：US12247198B2

公开(公告)日：2025-03-11

申请号：US17311697

申请日：2018-12-14

Applicant: BGI SHENZHEN

Inventor： Xin Fang ,  Xiaoluo Huang ,  Yue Shen ,  Xun Xu

IPC: C12N15/10 ,  B01J19/00

Abstract: A nucleic acid synthesis device and a nucleic acid purification device, uses thereof, and a nucleic acid synthesis method and a nucleic acid purification method. The nucleic acid synthesis device includes a solid support, and the solid support includes a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is attachable, though a covalent bond, to a phosphoramidite-protected nucleotide monomer or multimer for synthesis of nucleic acid. The nucleic acid synthesis device of the present disclosure can be used for not only synthesis of an oligonucleotide primer, but also for purification of enzymatic digestion and PCR product by using the oligonucleotide primer immobilized on the CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.