公开(公告)号：US20210116369A1

公开(公告)日：2021-04-22

申请号：US17077643

申请日：2020-10-22

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： EVAN THRUSH ,  NING LIU ,  CARL MARLOWE ,  WILLIAM BRADLEY STRONG

IPC: G01N21/552 ,  G06T7/00

Abstract: The invention relates to methods for characterizing the binding interactions between binding partners. The invention pertains to methods comprising contacting a first binding partner with a second binding partner that exhibits fast-off rate binding characteristics with the first binding partner to generate the binding interaction between the first binding partner and the second binding partner, wherein the first binding partner is immobilized onto a substrate that is designed to enhance the fluorescence signal of fluorescent molecules located near the substrate's surface, and wherein the second binding partner is a molecule that emits a fluorescent signal or is conjugated to molecule that emits a fluorescent signal. The interactions between the two binding partners can be analyzed based on multiple transient interactions between the two binding partners.

公开(公告)号：US20230111586A1

公开(公告)日：2023-04-13

申请号：US17964071

申请日：2022-10-12

Applicant: BIO-RAD LABORATORIES, INC. ,  ALIGHT SCIENCES INC.

Inventor： WILLIAM BRADLEY STRONG ,  ALEXANDER EDMUND JOHNSON-BUCK

IPC: B01L3/00 ,  G01N21/64

Abstract: This disclosure pertains to a consumable product (microplate) suitable for use in assays utilizing single-molecule recognition through equilibrium Poisson sampling (SiMREPS) and in other assays employing total internal reflection fluorescence (TIRF) or HiLo microscopy. The disclosed microplate is also suitable for use in other high throughput assay systems, such as single-molecule FRET, ligand-receptor binding studies, membrane biology assays, cell-based TIRF and near-TIRF assays. The disclosure further pertains to the use of the microfluidic microplate for the detection of analytes, including nucleic acids, polypeptides, carbohydrates, lipids, post-translational modifications, amino acids, metabolites, and small molecules.