公开(公告)号：US20020082416A1

公开(公告)日：2002-06-27

申请号：US10029281

申请日：2001-12-28

申请人： BIONEER CORPORATION

发明人： Han Oh Park ,  In Woo Lee

IPC分类号： C07H021/04

CPC分类号： C12N15/1003

摘要： A method for obtaining DNA from fish spermatogonia comprises (i) disrupting fish spermatogonium to obtain a milky-white colloid, (ii) adding an alkaline solution of pH 8 to pH 12, containing more than 1 mol of salts, such as monovalent salts, to the milky-white colloid, to separate DNA from protamines, and then (iii) adding ethanol solution to the resultant mixture, effecting a precipitation of DNA.

摘要翻译： 从鱼类精原细菌获得DNA的方法包括：（i）破碎鱼类精母细胞以获得乳白色胶体，（ii）将pH8的碱性溶液加入pH12，含有多于1摩尔的盐，如一价盐， 向乳白色胶体中分离DNA，然后（iii）向所得混合物中加入乙醇溶液，进行DNA的沉淀。