专利检索 ap:("BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM") AND inv:"Vahid AFSHAR-KHARGHAN" 第 1 页

1.

发明公开
PRODUCTION OF MEGAKARYOCYTES AND PLATELETS IN A CO-CULTURE SYSTEM 审中-公开

公开(公告)号：US20230383257A1

公开(公告)日：2023-11-30

申请号：US18248193

申请日：2021-10-15

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： Elizabeth SHPALL , Katy REZVANI , Bijender KUMAR , Mayela MENDT , Vahid AFSHAR-KHARGHAN , Rafet BASAR

IPC分类号： C12N5/078 , A61K35/19

CPC分类号： C12N5/0644 , A61K35/19 , C12N2506/1369 , C12N2502/11 , C12N2501/727 , C12N2501/125 , C12N2501/145 , C12N2501/2306 , C12N2501/724

摘要： Embodiments of the disclosure include systems, methods, and compositions for producing megakaryocytes and platelets for recipient individuals in need thereof. The megakaryocytes and platelets are produced following co-culture of MSCs and CD34+ cells in media comprising stem cell factor, thrombopoietin, and IL-6, and wherein at least the CD34+ cells have a knock-in of HLA-E at the beta-2-microglobulin genomic locus, in specific embodiments. In some cases, ROCK inhibitors are utilized.