公开(公告)号：US3806420A

公开(公告)日：1974-04-23

申请号：US24958872

申请日：1972-05-02

申请人： BOEHRINGER MANNHEIM GMBH

发明人： HOLZ G ,  GRAMSALL J ,  NELBOECK HOCHSTETTER M ,  BERGMEYER H

IPC分类号： C12N9/78 ,  C12N9/86 ,  C12Q1/34 ,  C12Q1/50 ,  C12D13/10

CPC分类号： C12Q1/34 ,  C12N9/78 ,  C12N9/86 ,  C12Q1/50 ,  G01N2333/9123 ,  G01N2333/978 ,  G01N2333/986 ,  Y10S435/815 ,  Y10S435/816 ,  Y10S435/824 ,  Y10S435/829 ,  Y10S435/933

摘要： CREATININE AMIDOHYDROLASE AND CREATINE AMIDINOHYDROLASE ARE PREPARED BY A PROCESS COMPRISING CULTURING (A) ALCALIGENCE SPEC. WS 51400 OR (B) PENICILLIUM WS 90001; IN A CREATININE-CONTAINING MEDIUM, DIGESTING SAME, OBTAINING THE CREATININE AMIDIODOHYDROLASE IN SUBSTANTIALLY PURE FORM FROM THE WATER-SOLUBLE DIGESTION FRACTION AND, OPTIONALLY SEPARATING BY ION EXCHANGE CHROMATOGRAPHY ANY CREATININE AMIDINOHYDROSALE PRESENT.