公开(公告)号：US20140107061A1

公开(公告)日：2014-04-17

申请号：US14052247

申请日：2013-10-11

Applicant: Bayer CropScience LP

Inventor： Damian CURTIS ,  Shaohua GUAN ,  Magalie GUILHABERT-GOYA ,  Jonathan S. MARGOLIS ,  Reed Nathan ROYALTY ,  Gerardo Bueno SALAZAR ,  David SESIN ,  Frisby Davis SMITH ,  Colleen TAYLOR ,  Hong ZHU

IPC: A01N43/54 ,  C12P19/38

CPC classification number: A01N43/54 ,  C12N1/20 ,  C12N15/01 ,  C12P19/385 ,  C12R1/465

Abstract: The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L.

Abstract translation: 本发明涉及新霉菌Streptomyces microflavus及其用于控制​​植物疾病或害虫的方法。 本发明还涉及通过培养产生Gougerotin的链霉菌菌株而获得的发酵液，其中发酵液含有至少约1g / L gougerotin。 本发明还涉及一种生产发酵木瓜霉菌菌株发酵液的方法，其中发酵液含有至少约1g / L gougerotin，该方法包括在含有可消化碳源的培养基中培养链霉菌属菌株， 在需氧条件下的可消化氮源，其中培养基含有浓度达到至少1g / L的gougerotin浓度的氨基酸。

公开(公告)号：US20140105862A1

公开(公告)日：2014-04-17

申请号：US14052094

申请日：2013-10-11

Applicant: Bayer CropScience LP

Inventor： Brian CAMPBELL ,  Damian CURTIS ,  Shaohua GUAN ,  Magalie GUILHABERT-GOYA ,  Daniel M. JOO ,  Tara LU ,  Jonathan S. MARGOLIS ,  Reed Nathan ROYALTY ,  Gerardo Bueno SALAZAR ,  David SESIN ,  Frisby Davis SMITH ,  Colleen TAYLOR ,  Hong ZHU

IPC: A01N63/02 ,  A01N43/54

CPC classification number: A01N63/02 ,  A01N43/54 ,  C12P19/28 ,  C12P19/385

Abstract: The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L. The present disclosure also relates to the molecular cloning of a gougerotin biosynthetic gene cluster from Streptomyces microflavus, and identification of individual genes in the gene cluster as well as the proteins encoded thereby. A gougerotin gene cluster comprising 13 open reading frames (ORFs) is located within a genetic locus of Streptomyces microflavus.

Abstract translation: 本发明涉及新霉菌Streptomyces microflavus及其用于控制​​植物疾病或害虫的方法。 本发明还涉及通过培养产生Gougerotin的链霉菌菌株而获得的发酵液，其中发酵液含有至少约1g / L gougerotin。 本发明还涉及一种生产发酵木瓜霉菌菌株发酵液的方法，其中发酵液含有至少约1g / L gougerotin，该方法包括在含有可消化碳源的培养基中培养链霉菌属菌株， 在需氧条件下的可消化氮源，其中培养基含有浓度达到至少1g / L的gougerotin浓度的氨基酸。 本公开还涉及从Streptomyces microflavus分子克隆gougerotin生物合成基因簇，以及鉴定基因簇中的各个基因以及由此编码的蛋白质。 包含13个开放阅读框（ORF）的gougerotin基因簇位于Streptomyces microflavus的遗传基因座内。