公开(公告)号：US20180364245A1

公开(公告)日：2018-12-20

申请号：US15780219

申请日：2016-12-06

Applicant: Becton, Dickinson and Company

Inventor： Jody MARTIN ,  Robert J. Radford ,  James Ghadiali

IPC: G01N33/58 ,  G01N33/542 ,  C07K16/44

Abstract: Fluorogenic sensors for a target analyte are provided. The fluorogenic sensors can include a water soluble light harvesting multichromophore and a fluorogenic dye covalently linked to the multichromophore and in energy-receiving proximity therewith. Aspects of the fluorogenic sensors include selective recognition of the target analyte by chemical reaction or by specific binding. In some cases, the linked fluorogenic dye includes a lactone or spiro-lactone group that is configured to chemically react with a target analyte via lactone ring-opening to produce a fluorescent dye configured for excitation by the multichromophore. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject fluorogenic sensors find use are provided. Systems, devices and kits for practicing the subject methods are provided.

公开(公告)号：US20240287591A1

公开(公告)日：2024-08-29

申请号：US18686812

申请日：2022-08-30

Applicant: Becton, Dickinson and Company

Inventor： Hye-Won SONG ,  Jody MARTIN ,  Margaret NAKAMOTO

IPC: C12Q1/6841 ,  C12Q1/6804 ,  G01N33/53 ,  G01N33/68

CPC classification number: C12Q1/6841 ,  C12Q1/6804 ,  G01N33/5308 ,  G01N33/6842 ,  C12Q2600/158 ,  C12Q2600/166 ,  G01N2333/958

Abstract: Disclosed herein include systems, methods, compositions, and kits for using decoy polynucleotides in noise reduction. Decoy oligonucleotides provided herein can, in some embodiments, prevent or reduce undesirable non-specific binding of intracellular target-binding reagent specific oligonucleotides or protein target-binding reagent specific oligonucleotides to non-target cellular/endogenous nucleic acids, and therefore reduce noise in methods provided herein, such as, for example, single cell multiomics analysis.