公开(公告)号：US10655117B2

公开(公告)日：2020-05-19

申请号：US16029647

申请日：2018-07-09

申请人： Beijing Jinhong Tianbang Information Technology Co., ltd

发明人： Yu Wang ,  Litong Ban ,  Liang Huang ,  Ning Sun ,  Hongpeng Yang

IPC分类号： C12N9/44 ,  C12N9/24 ,  C12N15/74 ,  C12N15/52 ,  C12R1/84 ,  C12P19/02 ,  C12P19/14 ,  C07H21/04

摘要： The present application relates to the field of enzyme engineering, especially relates to a pullulanase as well as preparation and use thereof. The pullulanase and coding gene thereof were obtained by random mutation by using the Error-prone PCR technique on the gene of wild-type pullulanase to obtain a mutant PLUM. The enzyme activity of the mutant PLUM was improved by 57.03% compared with the wild-type pullulanase PLUM.