公开(公告)号：US5190869A

公开(公告)日：1993-03-02

申请号：US706333

申请日：1991-05-28

申请人： Bert Rehr ,  Hermann Sahm

发明人： Bert Rehr ,  Hermann Sahm

IPC分类号： C12P7/18 ,  C12P7/58

CPC分类号： C12P7/58 ,  C12P7/18 ,  Y10S435/822

摘要： The long-term activity of the biocatalyst for the production of sorbitol and gluconic acid from an aqueous solution of fructose and glucose is considerably improved with the aid of permeabilized cells of Zymomonas mobilis immobilized using .kappa.-carrageenan that has been rigidified and then stabilized by K.sup.+ ions. Rigidification of .kappa.-carrageenan is preferably carried out by treatment with glutaraldehyde alone or by treatment with polyethyleneimine or hexamethylenediamine and subsequent exposure to glutaraldehyde. A buffer-free solution is preferred and the pH is maintained by adding Ca.sup.++ ions while simultaneously precipitating gluconic acid.

摘要翻译： 借助于使用κ-卡拉胶来固定的运动发酵单胞菌的透化细胞，通过K + - 角叉菜胶固化，然后通过K +稳定化，生物催化剂从果糖和葡萄糖水溶液中生产山梨糖醇和葡萄糖酸的长期活性显着提高 离子。 κ-卡拉胶的刚性优选通过单独使用戊二醛处理或通过用聚乙烯亚胺或六亚甲基二胺处理，随后暴露于戊二醛进行。 优选无缓冲溶液，通过加入Ca ++离子同时沉淀葡萄糖酸来维持pH。

公开(公告)号：US5102795A

公开(公告)日：1992-04-07

申请号：US606821

申请日：1990-10-31

申请人： Bert Rehr ,  Hermann Sahm

发明人： Bert Rehr ,  Hermann Sahm

IPC分类号： C12P7/18 ,  C12P7/58

CPC分类号： C12P7/18 ,  C12P7/58 ,  Y10S435/822

摘要： A process for obtaining sorbitol and gluconic acid or gluconate from aqueous glucose/fructose mixtures by using Zymomonas mobilis cells which have been permeabilized by treatment with cationic surfactant is disclosed. The permeabilized cells are preferably obtained by treatment with long-chain quaternary alkylammonium salts such as, in particular, CTAB, Dodigen or Bardac. Surfactant concentrations of 0.1 to 0.3% and a treatment time of 1 to 10 minutes at room temperature are expedient. The cell concentrations preferably used for the fermentation are 20 to 80 g/1 dry matter of permeabilized cells.

摘要翻译： 公开了通过使用通过用阳离子表面活性剂处理透化的运动发酵单胞菌细胞从水性葡萄糖/果糖混合物中获得山梨糖醇和葡萄糖酸或葡萄糖酸盐的方法。 透化细胞优选通过用长链季铵烷基铵盐，特别是CTAB，Dodigen或Bardac处理来获得。 表面活性剂浓度为0.1〜0.3％，室温下处理时间为1〜10分钟为宜。 优选用于发酵的细胞浓度为透化细胞的20〜80g / l干物质。