公开(公告)号：US20190218593A1

公开(公告)日：2019-07-18

申请号：US16215509

申请日：2018-12-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin R. CHIA ,  Amy L. HIDDESSEN ,  Benjamin J. HINDSON ,  Adam LOWE ,  Chunxiao HAN

IPC: C12Q1/686 ,  C08G77/46 ,  B01F17/54

CPC classification number: C12Q1/686 ,  B01F17/0071 ,  C07F7/1804 ,  C08G77/46 ,  C08G77/50 ,  C08G77/70 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.