公开(公告)号：US20230182127A1

公开(公告)日：2023-06-15

申请号：US18076667

申请日：2022-12-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal Sharma ,  Ronald Lebofsky ,  Man Cheng ,  Adam McCoy ,  Kalyan Handique ,  Eric Pomaranski

IPC: B01L3/00 ,  C12N13/00

CPC classification number: B01L3/502 ,  C12N13/00 ,  B01L2200/026 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/0893

Abstract: Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

公开(公告)号：US20210171939A1

公开(公告)日：2021-06-10

申请号：US17109704

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam McCoy ,  Kalyan Handique ,  Jonathan Bell ,  Vishal Sharma ,  Sida Wang

IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

Abstract: An embodiment of a composition for target material separation includes: a body and one or more molecules coupled to the body and structured for functionalization of the composition. In embodiments, each of the one or more molecules can include one or more of: a linker region; a polymerase chain reaction (PCR) segment or oligo binding region; one or more barcode region(s); a unique molecule identifier; a preparation-facilitating segment; an active segment; and a molecular scissor or cleavage region, wherein various regions can be coupled together in order to provide functionality to the composition. The invention(s) also cover manufacturing of the composition and various applications of use, in the context of target material capture (e.g., from single cells or other biological material).