公开(公告)号：US20200318106A1

公开(公告)日：2020-10-08

申请号：US16955682

申请日：2018-12-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Alex Michael WARD ,  Molly Mo-Yin LEUNG ,  Jean Frances SPAGNOLO-SMITH ,  George CHENAUX

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/113 ,  C12N15/72

Abstract: Engineered microbial organisms for altering gene expression are provided. In some embodiments, the engineered microbial organism comprises: one or more heterologous polynucleotide sequence comprising a phenotype coding sequence operably linked to a promoter; a heterologous polynucleotide sequence comprising an inducible promoter operably linked to a polynucleotide encoding a Cas nuclease; and a heterologous polynucleotide sequence comprising a guide RNA (gRNA) that targets the phenotype coding sequence.