公开(公告)号：US20180258455A1

公开(公告)日：2018-09-13

申请号：US15977254

申请日：2018-05-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song GONG ,  John SULLIVAN ,  Rongdian FU ,  Yan WANG ,  Evan H. BURSEY ,  Man CHENG

IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6804 ,  C07K16/40 ,  C12N9/12

CPC classification number: C12P19/34 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6804 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/101

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.