公开(公告)号：US12123888B2

公开(公告)日：2024-10-22

申请号：US16878549

申请日：2020-05-19

申请人： Isolation Bio Inc.

发明人： Peter Christey ,  Alexander Hallock

IPC分类号： G01N35/10 ,  B01L3/00 ,  B01L3/02 ,  C12M1/04 ,  C12M1/26 ,  C12M1/32 ,  C12Q1/6853 ,  G01N35/00 ,  G06T7/73

CPC分类号： G01N35/1081 ,  B01L3/5085 ,  C12M23/12 ,  C12M23/24 ,  C12M33/04 ,  C12Q1/6853 ,  G01N35/00029 ,  G01N35/00693 ,  G01N35/1004 ,  G06T7/74 ,  B01L3/0244 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2300/021 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/165 ,  G01N2035/00148 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30204 ,  G06T2207/30232 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2547/101 ,  C12Q2563/159 ,  C12Q2563/179

摘要： A picking instrument for picking biological samples, such as microbial samples, includes a picking pin having a distal tip and three degrees-of-freedom and configured to move in x, y, and z directions. The picking instrument also includes a loading platform comprising a first area and a second area, the first area configured to accommodate and secure a microfabricated chip including a plurality of microwells. The picking pin is programmatically controlled to pick a sample contained in one or more selected microwells of the microfabricated chip and then transfer the sample to a predetermined location at the destination sample holder. Methods of operating the picking instrument, including calibrating the coordinates of the selected microwell(s) relative to a location of the picking pin, are also provided.

公开(公告)号：US12104212B2

公开(公告)日：2024-10-01

申请号：US18505026

申请日：2023-11-08

申请人： Myriad Women's Health, Inc.

发明人： Jared Robert Maguire ,  Clement S. Chu ,  Imran Saeedul Haque ,  Eric Andrew Evans ,  Noah Welker

IPC分类号： C12Q1/6886 ,  C12Q1/6818 ,  C12Q1/6874

CPC分类号： C12Q1/6886 ,  C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6818 ,  C12Q2521/113 ,  C12Q2521/327 ,  C12Q2531/113

摘要： The present disclosure relates to a laboratory execution system that provides for automation of laboratory processes. A centralized data management system may be dynamically updated and used to facilitate management of components of the laboratory execution system, such as an automation system and an analytics results management system that may facilitate complex analytical functions, such as synthesizing raw test data. Potential workflows include the detection of specific molecules of interest.

公开(公告)号：US20240294993A1

公开(公告)日：2024-09-05

申请号：US18439529

申请日：2024-02-12

申请人： MICROBIAL DISCOVERY GROUP, LLC ,  UNITED ANIMAL HEALTH, INC.

发明人： Michael R. KING ,  Sona SON ,  Joel D. SPENCER ,  Amy LANGE ,  Jessica EDWARD ,  Ardean VELDKAMP

IPC分类号： C12Q1/689 ,  C12M1/00 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6851

CPC分类号： C12Q1/689 ,  C12M45/22 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6851 ,  C12Q2531/113 ,  C12Q2545/113 ,  C12Q2565/625

摘要： This invention relates to a method of detecting a gene, a method of determining the expression level of a gene, and compositions for use in these methods.

公开(公告)号：US20240287581A1

公开(公告)日：2024-08-29

申请号：US18652095

申请日：2024-05-01

申请人： Telesis Bio, Inc.

发明人： John E. Gill ,  Lixia Fu ,  Daniel G. Gibson

IPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12Q1/6862

CPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12Q1/6862 ,  C12Q2521/501 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Y605/00

摘要： The invention provides methods of assembling a DNA molecule having a desired sequence. The methods involve contacting a DNA ligase with a plurality of short oligonucleotides to be assembled and performing the ligase chain reaction to thereby generate a set of polynucleotides. Oligonucleotides in the plurality overlap with and are complementary to a sequence of at least one other oligonucleotide in the plurality, and at least 50% of the oligonucleotides in the plurality are 6-30 nucleotides in length. The set of polynucleotides produced are contacted with a DNA polymerase and dNTPs in a mixture to join the set of polynucleotides and thereby create a DNA molecule having a desired sequence by polymerase chain assembly. The method allows for production of oligonucleotides of any length having very high sequence fidelity to a desired sequence.

公开(公告)号：US12018316B2

公开(公告)日：2024-06-25

申请号：US17185609

申请日：2021-02-25

申请人： CODEX DNA, INC.

发明人： John E. Gill ,  Lixia Fu ,  Daniel G. Gibson

IPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12Q1/6862

CPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12Q1/6862 ,  C12Q2521/501 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Y605/00

摘要： The invention provides methods of assembling a DNA molecule having a desired sequence. The methods involve contacting a DNA ligase with a plurality of short oligonucleotides to be assembled and performing the ligase chain reaction to thereby generate a set of polynucleotides. Oligonucleotides in the plurality overlap with and are complementary to a sequence of at least one other oligonucleotide in the plurality, and at least 50% of the oligonucleotides in the plurality are 6-30 nucleotides in length. The set of polynucleotides produced are contacted with a DNA polymerase and dNTPs in a mixture to join the set of polynucleotides and thereby create a DNA molecule having a desired sequence by polymerase chain assembly. The method allows for production of oligonucleotides of any length having very high sequence fidelity to a desired sequence.

公开(公告)号：US11965212B2

公开(公告)日：2024-04-23

申请号：US17500858

申请日：2021-10-13

申请人： Quest Diagnostics Investments LLC

发明人： Heather R. Sanders ,  Kevin Z. Qu ,  Charles M. Strom ,  Richard A. Bender

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q1/6886 ,  C12Q2531/113 ,  C12Q2521/301

摘要： Provided herein are methods and compositions for detection of a nucleic acid target in a sample. The methods and compositions use primer directed amplification in conjunction with nucleic acid fragmentation. The methods have high sensitivity even in the presence of a large amount of non-target nucleic acid. Also provided are oligonucleotides and kits useful in the method. Exemplary nucleic acid targets are those with mutant gene sequence such as mutant sequence of the EGFR, APC, TMPRSS2, ERG and ETV1 genes.

公开(公告)号：US11939626B2

公开(公告)日：2024-03-26

申请号：US17813415

申请日：2022-07-19

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Daniel T. Chiu ,  Jason E. Kreutz ,  Gloria S. Yen ,  Bryant S. Fujimoto

IPC分类号： C12Q1/6816 ,  G01N15/14 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/64 ,  G06T7/62 ,  G06V20/69

CPC分类号： C12Q1/6816 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/6428 ,  G06T7/62 ,  G06V20/695 ,  G01N2015/1445 ,  G01N2021/6439 ,  G06T2207/10056 ,  G06T2207/30072 ,  C12Q1/6816 ,  C12Q2531/113 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2563/159

摘要： Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

公开(公告)号：US11932910B2

公开(公告)日：2024-03-19

申请号：US17383273

申请日：2021-07-22

申请人： Myriad Women's Health, Inc.

发明人： Jared Robert Maguire ,  Clement S. Chu ,  Imran Saeedul Haque ,  Eric Andrew Evans ,  Noah Welker

IPC分类号： C12Q1/6874 ,  C12Q1/6818 ,  C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6818 ,  C12Q2521/113 ,  C12Q2521/327 ,  C12Q2531/113

摘要： The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

公开(公告)号：US20240043916A1

公开(公告)日：2024-02-08

申请号：US18361786

申请日：2023-07-28

申请人： GEN-PROBE INCORPORATED

发明人： Jennifer L. TIDD ,  Ann-Laure SHAPIRO ,  Ankur H. SHAH ,  James T. TUGGLE ,  David OPALSKY ,  Alberto A. LIO ,  Timothy J. SCHEER ,  Jason F. RHUBOTTOM ,  David Aaron BUSE

IPC分类号： C12Q1/6844

CPC分类号： C12Q1/6844 ,  C12Q2523/32 ,  C12Q2527/101 ,  C12Q2531/113 ,  C12Q2565/629

摘要： A method of performing a lab developed test for detecting a nucleic acid analyte on an automated analyzer. The method includes a first step of using a computer to select, define or modify one or more user-defined parameters of a protocol for performing the lab developed test on the analyzer, where each user-defined parameter of the protocol defines a step to be performed by the analyzer during the lab developed test. The method further includes a second step of performing the lab developed test with the protocol of the first step, where the analyzer stores one or more system-defined parameters for performing the lab developed test, the one or more system-defined parameters being installed on the analyzer prior to performing first step.

公开(公告)号：US11795492B2

公开(公告)日：2023-10-24

申请号：US16919238

申请日：2020-07-02

申请人： ArcherDX, LLC

发明人： Joshua Stahl ,  Jason Myers ,  Brady Culver ,  Brian Kudlow

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/6855

CPC分类号： C12Q1/6806 ,  C12N15/1096 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q2525/117 ,  C12Q2549/119 ,  C12Q1/6806 ,  C12Q2521/101 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2531/113 ,  C12Q2563/131 ,  C12Q2563/143

摘要： Aspects of the technology disclosed herein relate to methods of preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.