公开(公告)号：US20250043362A1

公开(公告)日：2025-02-06

申请号：US18793760

申请日：2024-08-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： George Karlin-Neumann ,  Ronald Lebofsky ,  Mona Shahbazian ,  Mina Rostamza

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/686 ,  G01N21/64

Abstract: The invention provides methods for the detection of molecular targets by digital PCR (dPCR) with a blocker for non-target molecules. Each target is provided with a unique mixture of probes. The blocker binds to a non-target molecule and blocks it from contributing to fluorescence from partitions. Two or more colors of fluorescence intensity are read, and two colors of fluorescence intensity are plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a blocker selects against the detection of non-target molecules and improves the resolution of radial multiplexing, allowing multiple targets to be detected.