公开(公告)号：US20240084374A1

公开(公告)日：2024-03-14

申请号：US18465321

申请日：2023-09-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Chenyu LI ,  Olga MIKHAYLICHENKO ,  Nathan HENDEL ,  Anthony HENRIQUEZ ,  Richard DANNEBAUM ,  Monica HERRERA ,  Eric HALL ,  Séverine MARGERIDON ,  Thea RIEL

IPC: C12Q1/686

CPC classification number: C12Q1/686 ,  C12Q2600/154

Abstract: The digital amplification methods and kits provide the ability to estimate the fetal fraction of cell-free DNA (cfDNA) in a maternal sample, e.g., plasma or serum, by analysis of target sites that are differentially methylated in fetal and maternal cfDNA.

公开(公告)号：US20240102086A1

公开(公告)日：2024-03-28

申请号：US18370566

申请日：2023-09-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Séverine MARGERIDON ,  Monica HERRERA ,  Richard DANNEBAUM ,  Nyaradzo DZVOVA ,  Raymond-John ABAYAN ,  Darren R. LINK

IPC: C12Q1/6853

CPC classification number: C12Q1/6853

Abstract: Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.