公开(公告)号：US12259356B2

公开(公告)日：2025-03-25

申请号：US17544591

申请日：2021-12-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kenneth Oh ,  Carl Marlowe ,  Stephen L. Swihart ,  Evan Thrush ,  Michael Griffin

IPC: G01N27/447 ,  G01N21/64 ,  G01N33/58 ,  G01N33/68

Abstract: Methods and reference compositions for quantifying protein using tagged standards. In an exemplary method, a reference composition and a protein may be electrophoresed in respective lanes of a gel. The reference composition may include quantitation standards of different size and each including a tag present at a different concentration. The quantitation standards and the protein may be transferred from the gel to a solid support to create a blot. Luminescence may be detected from the blot to obtain respective luminescence values separately representing an abundance of the tag of each quantitation standard and an abundance of the protein. A quantity of the protein may be determined using the respective luminescence values.