公开(公告)号：US20230323435A1

公开(公告)日：2023-10-12

申请号：US18185220

申请日：2023-03-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal SHARMA ,  Ronald LEBOFSKY

IPC: C12Q1/6837 ,  C12N15/10

CPC classification number: C12Q1/6837 ,  C12N15/1065

Abstract: The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.