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公开(公告)号：US10421991B2
公开(公告)日：2019-09-24
申请号：US14938242
申请日：2015-11-11
IPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q1/689
摘要： Methods for typing a strain of an organism are provided, the methods comprising the steps of amplifying, in a single reaction mixture containing nucleic acid from the organism, dividing the reaction mixture into a plurality of sets of second-stage reaction wells, each set of second-stage reaction wells containing a different pair of second-stage primers, subjecting each of the second-stage reaction wells to amplification conditions to generate a plurality of second-stage amplicons, melting the second-stage amplicons to generate a melting curve for each second-stage amplicon, and identifying the strain of the organism from the melting curves.
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公开(公告)号：US10724081B2
公开(公告)日：2020-07-28
申请号：US15660609
申请日：2017-07-26
摘要： Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance.
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公开(公告)号：US20160153034A1
公开(公告)日：2016-06-02
申请号：US14938242
申请日：2015-11-11
IPC分类号： C12Q1/68
摘要： Methods for typing a strain of an organism are provided, the methods comprising the steps of amplifying, in a single reaction mixture containing nucleic acid from the organism, dividing the reaction mixture into a plurality of sets of second-stage reaction wells, each set of second-stage reaction wells containing a different pair of second-stage primers, subjecting each of the second-stage reaction wells to amplification conditions to generate a plurality of second-stage amplicons, melting the second-stage amplicons to generate a melting curve for each second-stage amplicon, and identifying the strain of the organism from the melting curves.
摘要翻译： 提供了用于分选生物体菌株的方法，所述方法包括以下步骤：在含有来自生物体的核酸的单一反应混合物中，将反应混合物分成多组第二级反应孔，每组 含有不同对的第二级引物的第二级反应孔，使每个第二级反应孔经受扩增条件以产生多个第二级扩增子，使第二级扩增子熔化以产生每个第二级扩增子的熔解曲线 第二级扩增子，并从熔解曲线鉴定生物体的菌株。
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公开(公告)号：US20180010167A1
公开(公告)日：2018-01-11
申请号：US15660609
申请日：2017-07-26
摘要： Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance.
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