公开(公告)号：US20160018410A1

公开(公告)日：2016-01-21

申请号：US14868575

申请日：2015-09-29

Applicant: Biodesix, Inc.

Inventor： Heinrich Röder ,  Senait Asmellash ,  Jenna Allen ,  Maxim Tsypin

IPC: G01N33/68 ,  H01J49/40 ,  H01J49/00

CPC classification number: G01N33/487 ,  G01N33/6851 ,  H01J49/0004 ,  H01J49/164 ,  H01J49/40

Abstract: A method of analyzing a biological sample, for example serum or other blood-based samples, using a MALDI-TOF mass spectrometer instrument is described. The method includes the steps of applying the sample to a sample spot on a MALDI-TOF sample plate and directing more than 20,000 laser shots to the sample at the sample spot and collecting mass-spectral data from the instrument. In some embodiments at least 100,000 laser shots and even 500,000 shots are directed onto the sample. It has been discovered that this approach, referred to as “deep-MALDI”, leads to a reduction in the noise level in the mass spectra and that a significant amount of additional spectral information can be obtained from the sample. Moreover, peaks visible at lower number of shots become better defined and allow for more reliable comparisons between samples.

Abstract translation: 描述了使用MALDI-TOF质谱仪器分析生物样品，例如血清或其它基于血液的样品的方法。 该方法包括以下步骤：将样品施加到MALDI-TOF样品板上的样品点上，并将20,000多次激光照射引导到样品点处的样品并从仪器收集质谱数据。 在一些实施例中，至少100,000次激光照射甚至50万次照射被引导到样品上。 已经发现，称为“深MALDI”的方法导致质谱中噪声水平的降低，并且可以从样品获得大量附加的光谱信息。 此外，在较少的镜头可见的峰值变得更好地定义并且允许样品之间更可靠的比较。

公开(公告)号：US20130320203A1

公开(公告)日：2013-12-05

申请号：US13836436

申请日：2013-03-15

Applicant: BIODESIX, INC.

Inventor： Heinrich Röder ,  Senait Asmellash ,  Jenna Allen ,  Maxim Tsypin

IPC: G01N33/487

CPC classification number: G01N33/487 ,  G01N33/6851 ,  H01J49/0004 ,  H01J49/164 ,  H01J49/40

Abstract: A method of analyzing a biological sample, for example serum or other blood-based samples, using a MALDI-TOF mass spectrometer instrument is described. The method includes the steps of applying the sample to a sample spot on a MALDI-TOF sample plate and directing more than 20,000 laser shots to the sample at the sample spot and collecting mass-spectral data from the instrument. In some embodiments at least 100,000 laser shots and even 500,000 shots are directed onto the sample. It has been discovered that this approach, referred to as “deep-MALDI”, leads to a reduction in the noise level in the mass spectra and that a significant amount of additional spectral information can be obtained from the sample. Moreover, peaks visible at lower number of shots become better defined and allow for more reliable comparisons between samples.

Abstract translation: 描述了使用MALDI-TOF质谱仪器分析生物样品，例如血清或其它基于血液的样品的方法。 该方法包括以下步骤：将样品施加到MALDI-TOF样品板上的样品点上，并将20,000多次激光照射引导到样品点处的样品并从仪器收集质谱数据。 在一些实施例中，至少100,000次激光照射甚至50万次照射被引导到样品上。 已经发现，称为“深MALDI”的方法导致质谱中噪声水平的降低，并且可以从样品获得大量附加的光谱信息。 此外，在较少的镜头可见的峰值变得更好地定义并且允许样品之间更可靠的比较。

公开(公告)号：US09279798B2

公开(公告)日：2016-03-08

申请号：US13836436

申请日：2013-03-15

Applicant: Biodesix, Inc.

Inventor： Heinrich Röder ,  Senait Asmellash ,  Jenna Allen ,  Maxim Tsypin

IPC: G01N33/487 ,  H01J49/16 ,  G01N33/68 ,  H01J49/00 ,  H01J27/24 ,  H01J49/40

CPC classification number: G01N33/487 ,  G01N33/6851 ,  H01J49/0004 ,  H01J49/164 ,  H01J49/40

Abstract: A method of analyzing a biological sample, for example serum or other blood-based samples, using a MALDI-TOF mass spectrometer instrument is described. The method includes the steps of applying the sample to a sample spot on a MALDI-TOF sample plate and directing more than 20,000 laser shots to the sample at the sample spot and collecting mass-spectral data from the instrument. In some embodiments at least 100,000 laser shots and even 500,000 shots are directed onto the sample. It has been discovered that this approach, referred to as “deep-MALDI”, leads to a reduction in the noise level in the mass spectra and that a significant amount of additional spectral information can be obtained from the sample. Moreover, peaks visible at lower number of shots become better defined and allow for more reliable comparisons between samples.

Abstract translation: 描述了使用MALDI-TOF质谱仪器分析生物样品，例如血清或其它基于血液的样品的方法。 该方法包括以下步骤：将样品施加到MALDI-TOF样品板上的样品点上，并将20,000多次激光照射引导到样品点处的样品并从仪器收集质谱数据。 在一些实施例中，至少100,000次激光照射甚至50万次照射被引导到样品上。 已经发现，称为“深MALDI”的方法导致质谱中噪声水平的降低，并且可以从样品获得大量附加的光谱信息。 此外，在较少的镜头可见的峰值变得更好地定义并且允许样品之间更可靠的比较。

公开(公告)号：US09606101B2

公开(公告)日：2017-03-28

申请号：US14868575

申请日：2015-09-29

Applicant: Biodesix, Inc.

Inventor： Heinrich Röder ,  Senait Asmellash ,  Jenna Allen ,  Maxim Tsypin

IPC: H01J49/40 ,  G01N33/487 ,  H01J49/16 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/487 ,  G01N33/6851 ,  H01J49/0004 ,  H01J49/164 ,  H01J49/40

Abstract: A method of analyzing a biological sample, for example serum or other blood-based samples, using a MALDI-TOF mass spectrometer instrument is described. The method includes the steps of applying the sample to a sample spot on a MALDI-TOF sample plate and directing more than 20,000 laser shots to the sample at the sample spot and collecting mass-spectral data from the instrument. In some embodiments at least 100,000 laser shots and even 500,000 shots are directed onto the sample. It has been discovered that this approach, referred to as “deep-MALDI”, leads to a reduction in the noise level in the mass spectra and that a significant amount of additional spectral information can be obtained from the sample. Moreover, peaks visible at lower number of shots become better defined and allow for more reliable comparisons between samples.