公开(公告)号：US12130217B2

公开(公告)日：2024-10-29

申请号：US17984934

申请日：2022-11-10

申请人： Wisconsin Alumni Research Foundation

发明人： Joshua Coon ,  Michael Westphall

IPC分类号： G01N1/42 ,  G01N1/36 ,  G01N15/14 ,  H01J37/26 ,  H01J37/28 ,  H01J49/16

CPC分类号： G01N1/42 ,  G01N1/36 ,  G01N15/1468 ,  H01J37/26 ,  H01J37/28 ,  H01J49/164 ,  H01J49/165 ,  H01J2237/31745

摘要： The present invention provides methods for controllably forming a layer of amorphous ice and other amorphous solids on a substrate, and also provides cryo-electron microscopy (cryo-EM) sample preparation methods and systems that utilize in vacuo formation of amorphous ice and other solids. Formation of the amorphous solid layer can be independent of the deposition of sample molecules to be analyzed using electron microscopy, and allows for the generation of a uniformly thick layer. Optionally, mass spectrometry instruments are used to generate and purify molecules deposited on the generated amorphous solid layer. The techniques and systems described herein can deliver near ideal cryo-EM sample preparation to greatly increase resolution, sensitivity, scope, and throughput of cryo-EM protein imaging, and therefore greatly impact the field of structural biology.

公开(公告)号：US12105049B2

公开(公告)日：2024-10-01

申请号：US17739251

申请日：2022-05-09

申请人： Bruker Daltonics GmbH & Co. KG

发明人： Andreas Haase

IPC分类号： G01N27/62 ,  G01N27/623 ,  H01J49/00 ,  H01J49/16 ,  H01J49/40

CPC分类号： G01N27/623 ,  H01J49/0031 ,  H01J49/164 ,  H01J49/40

摘要： Disclosed is a method for analytically measuring sample material deposited on a sample support surface, comprising: (a) defining a plurality of regions on the surface, several of which are in contact with sample material, (b1) sampling sections of sample on a region using a desorbing beam to generate desorbed molecules, which are ionized and transferred to an analyzer, (b2) in so doing, sweeping the region by changing an orientation setting of the beam relative to the surface along a non-rectilinear trajectory on the region selected from a plurality of predefined, non-rectilinear trajectories while keeping the support in one position, (c) transitioning from a swept region to a region to be swept next using spatial adjustment of the support, and (d) repeating steps (b1), (b2), and (c) until a predetermined termination condition is fulfilled. A system for analyzing ions, having an ion generation device and a control unit is also disclosed.

公开(公告)号：US20240290602A1

公开(公告)日：2024-08-29

申请号：US18574591

申请日：2022-06-22

申请人： SHIMADZU CORPORATION

发明人： Kanae TERAMOTO

IPC分类号： H01J49/16

CPC分类号： H01J49/164

摘要： A microorganism mass spectrometry method includes: forming, on a sample plate, a mixed crystal of a matrix material and a microorganism sample which is a sample including a test microorganism or a component derived from the test microorganism; performing, in a mass spectrometer for performing analysis through matrix-assisted laser desorption/ionization mass spectrometry, a first laser irradiation in which a predetermined micro region on the mixed crystal is irradiated with a laser beam for a predetermined number of times; performing, in the mass spectrometer, a second laser irradiation in which the micro region or a predetermined region in the micro region is irradiated with the laser beam for a predetermined number of times; and generating mass spectrometry data indicating a mass spectrometry result for the mixed crystal, on the basis of ion detection signals acquired by the mass spectrometry device during the second laser irradiation.

公开(公告)号：US12057304B2

公开(公告)日：2024-08-06

申请号：US17337919

申请日：2021-06-03

申请人： UT-Battelle, LLC

发明人： Olga S. Ovchinnikova ,  Anton V. Ievlev ,  Matthias Lorenz ,  Nikolay Borodinov ,  Steven T. King

IPC分类号： H01J49/00 ,  G01N23/2258 ,  G06N5/04 ,  G06N20/00 ,  H01J49/16

CPC分类号： H01J49/0036 ,  G01N23/2258 ,  G06N5/04 ,  G06N20/00 ,  H01J49/0004 ,  H01J49/164 ,  G01N2223/07 ,  G01N2223/40

摘要： Machine learning approach can combine mass spectral imaging (MSI) techniques, one with low spatial resolution but intact molecular spectra and the other with nanometer spatial resolution but fragmented molecular signatures, to predict molecular MSI spectra with submicron spatial resolution. The machine learning approach can perform transformations on the spectral image data of the two MSI techniques to reduce dimensionality, and using a correlation technique, find relationships between the transformed spectral image data. The determined relationships can be used to generate MSI spectra of desired resolution.

公开(公告)号：US12040452B2

公开(公告)日：2024-07-16

申请号：US17261516

申请日：2019-07-19

申请人： Dyson Technology Limited

发明人： Michael Edward Rendall

IPC分类号： H01M10/0585 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10 ,  H01J49/16

CPC分类号： H01M10/0585 ,  H01J49/0004 ,  H01J49/04 ,  H01J49/105 ,  H01J49/164

摘要： A method comprises obtaining a stack for an energy storage device, the stack comprising one or more layers: laser ablating the stack so as to form a cut through one or more of the layers, thereby producing one or more laser ablation products: and analysing, using a mass spectrometry-based analysis technique, the laser ablation products thereby to determine one or more characteristics of the stack. Also disclosed is an apparatus.

公开(公告)号：US12031943B2

公开(公告)日：2024-07-09

申请号：US17626535

申请日：2019-07-19

申请人： Shimadzu Corporation ,  National Institute of Advanced Industrial Science and Technology

发明人： Hidenori Takahashi ,  Daiki Asakawa

IPC分类号： G01N27/62 ,  H01J49/00 ,  H01J49/02 ,  H01J49/42 ,  H01J49/16 ,  H01J49/24 ,  H01J49/40

CPC分类号： G01N27/62 ,  H01J49/0036 ,  H01J49/0068 ,  H01J49/025 ,  H01J49/424 ,  H01J49/164 ,  H01J49/24 ,  H01J49/40

摘要： An ion analyzer for analyzing product ions generated by irradiating precursor ions derived from a sample component with radicals, the ion analyzer including a reaction chamber 2, a radical supply unit 5, 6 configured to generate radicals and supply the radicals to the reaction chamber, a radical temperature acquisition unit 911, 912 configured to acquire a temperature of the radicals to be supplied to the reaction chamber, a standard substance supply unit 11 configured to supply a predetermined amount of predetermined precursor ions to the reaction chamber, the predetermined precursor ions being generated from a standard substance whose activation energy of a reaction in which the radicals attach to the standard substance is known, an ion measurement unit 92 configured to measure an amount of predetermined product ions generated from the precursor ions derived from the standard substance by the reaction with the radicals, a reactive radical amount calculation unit 93 configured to obtain an amount of reactive radicals based on the amount of the predetermined product ions, and a radical density calculation unit 94 configured to obtain a radical density based on the temperature of the radicals, the activation energy, and the amount of the reactive radicals.

公开(公告)号：US11988683B2

公开(公告)日：2024-05-21

申请号：US17017853

申请日：2020-09-11

申请人： SHIMADZU CORPORATION

发明人： Mio Tsukahara ,  Kenta Terashima ,  Yoshitake Yamamoto ,  Daisuke Oda

IPC分类号： G01N35/10 ,  H01J49/16

CPC分类号： G01N35/1002 ,  G01N35/1011 ,  H01J49/164

摘要： A moving mechanism and a spray mechanism are controlled to spray a reagent from a nozzle to a sample plate while relatively moving the sample plate and the nozzle. The reagent is applied to an entire application area by moving a spray spot of the reagent sprayed from the nozzle, on the sample plate from a start point to an end point. At this time, the spray spot is moved in a sample placement area after a spray amount of the reagent from the nozzle and a moving speed of the spray spot become constant.

公开(公告)号：US11988680B2

公开(公告)日：2024-05-21

申请号：US17376776

申请日：2021-07-15

申请人： SHIMADZU CORPORATION

发明人： Tomonori Oshikawa ,  Yuko Kobayashi ,  Yoshiki Tainaka ,  Tatsuki Okubo ,  Kohei Suzuki

IPC分类号： G01N35/00 ,  G01N33/49 ,  H01J49/16 ,  H01J49/40

CPC分类号： G01N35/00871 ,  G01N33/49 ,  H01J49/164 ,  H01J49/40 ,  G01N2035/00891

摘要： In a device (4) for assisting in analytical operations for correctly arranging samples in a plurality of sample-placement portions and implementing multiple protocols in the correct order, a storage section (41) holds sample information and analysis parameters for each protocol as well as the order of implementation. A protocol-selection-input receiver (42) receives an input of the selection of a protocol. A sample-position displayer (43) displays, on a display unit (6), sample information in the selected protocol along with the positions of the sample-placement portions. A protocol-implementation-information collector (45) collects information concerning the state of implementation of the protocols. A determiner (46) determines whether a protocol which must be implemented earlier was already implemented, based on the state of implementation and order of implementation of the protocols. A batch-file creator (47) creates a batch file when it is confirmed that the protocol which must be implemented earlier was already implemented.

公开(公告)号：US20240145227A1

公开(公告)日：2024-05-02

申请号：US18410961

申请日：2024-01-11

申请人： Roche Diagnostics Operations, Inc.

发明人： Martin Rempt ,  Manuel Josef Seitz ,  Christoph Zuth

IPC分类号： H01J49/16 ,  G01N27/623

CPC分类号： H01J49/164 ,  G01N27/623

摘要： A target (110) for use in a laser desorption mass spectrometer is disclosed. The target (110) has at least one surface (112). wherein the surface (112) is covered at least partially with at least one layer (114), wherein the layer (114) is a hydrogen comprising, silicon-incorporated amorphous carbon (a-C:H:Si) layer (116), wherein the a-C:H:Si layer (116) comprises:



40 at. % to 80 at. % of carbon;
1 at. % to 20 at. % of hydrogen; and
10 at. % to 40 at. % of silicon.

公开(公告)号：US20240096613A1

公开(公告)日：2024-03-21

申请号：US18467553

申请日：2023-09-14

申请人： Thermo Fisher Scientific (Bremen) GmbH

发明人： Hamish STEWART

IPC分类号： H01J49/06 ,  H01J49/16

CPC分类号： H01J49/065 ,  H01J49/164

摘要： An ion guide is provided for a spectrometer. The ion guide comprises a first electrode assembly, comprising a plurality of guide electrodes aligned along a central axis. Each guide electrode comprises an aperture. The apertures define an ion channel from a first end of the first electrode assembly to a second end of the first electrode assembly. The ion channel is configured to receive an ion beam at the first end. The guide electrodes are configured to receive RF voltages to define a confinement volume for the ion beam, wherein the confinement volume is centred about the central axis. The ion guide comprises a second electrode assembly adjacent to the first electrode assembly, wherein the second electrode assembly is configured to receive a DC voltage, such that a centre of the ion beam is deflected to be off the central axis.