公开(公告)号：US20150075261A1

公开(公告)日：2015-03-19

申请号：US14391775

申请日：2013-04-11

Applicant: Biogen Idec MA Inc.

Inventor： Zoran Sosic ,  Andrew Blum ,  Bing Guan ,  Boris Boumajny

IPC: G01N30/60

CPC classification number: G01N30/60 ,  C08B37/0003 ,  C08B37/0021 ,  C08B37/0063 ,  G01N33/6842 ,  G01N2030/027 ,  G01N2400/40

Abstract: In one aspect, the disclosure provides methods of distinguishing a glycosaminoglycan from one or more other components in a sample by subjecting the sample to size-exclusion chromatography using a mobile phase having a pH of 6.8 or lower. A mobile phase having a pH of 6.8 or lower is found to improve the separation of glycosaminoglycans from proteins during size exclusion chromatography. In some embodiments, improved separation is due to the low pH of the mobile phase causing elution of less dispersed fractions of the protein and/or glycosaminoglycan. In some embodiments, the overlap between protein and/or glycosaminoglycan fractions is reduced.

Abstract translation: 一方面，本公开提供了通过使用pH为6.8或更低的流动相对样品进行尺寸排阻色谱法来区分糖胺聚糖与样品中一种或多种其它组分的方法。 发现pH为6.8或更低的流动相可改善在尺寸排阻层析期间糖胺聚糖与蛋白质的分离。 在一些实施方案中，改进的分离是由于流动相的低pH导致较少分散的蛋白质和/或糖胺聚糖部分的洗脱。 在一些实施方案中，蛋白质和/或糖胺聚糖级分之间的重叠减少。