公开(公告)号：US20130065219A1

公开(公告)日：2013-03-14

申请号：US13659655

申请日：2012-10-24

Applicant: Biogen Idec MA Inc.

Inventor： Valerie Liu TSANG ,  Angela Xiaoying WANG ,  Helena YUSUF-MAKAGIANSAR

IPC: C12Q3/00

CPC classification number: C12P21/02 ,  C07K16/00 ,  Y10T436/115831 ,  Y10T436/116664 ,  Y10T436/117497 ,  Y10T436/12

Abstract: The present invention pertains to methods of increasing the efficiency of producing a bioproduct. In some embodiments, the method increases the quantity of a bioproduct produced, or decreases bioproduct production time, in a bioreactor cell culture producing the bioproduct, the method comprising, (a) intermittently or continuously analyzing the concentration of one or more nutrients in the bioreactor cell culture; and (b) adding to the bioreactor cell culture additional nutrient media when the concentration of the one or more nutrients is lower than a target value.

Abstract translation: 本发明涉及提高生物制品生产效率的方法。 在一些实施方案中，该方法在产生生物产物的生物反应器细胞培养物中增加产生的生物产物的量或减少生物产物生产时间，该方法包括：（a）间歇地或连续地分析生物反应器中一种或多种营养物质的浓度 细胞培养; 和（b）当一种或多种营养素的浓度低于目标值时，向生物反应器细胞培养添加另外的营养培养基。