公开(公告)号：US20220195492A1

公开(公告)日：2022-06-23

申请号：US17643161

申请日：2021-12-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: C12Q1/68 ,  G01N1/30 ,  G01N1/36 ,  G01N21/64

Abstract: Disclosed herein is a method for the detection of the presence of sperm DNA fragmentation in a semen sample. The method comprises a step of embedding the semen sample containing sperm cells in a gel comprising acrylamide, acrylic acid, methacrylic acid, N-isopropylacrylamide (NIPAM), alginate, or polyethylene glycol (PEG), to obtain a sperm cells-embedded gel. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US20230075204A1

公开(公告)日：2023-03-09

申请号：US18047242

申请日：2022-10-17

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: C12Q1/68 ,  C12Q1/6806 ,  G01N1/30 ,  G01N21/64

Abstract: Disclosed herein is a method for the detection of the presence of sperm DNA fragmentation in a semen sample. The method comprises a step of embedding the semen sample containing sperm cells in a gel comprising acrylamide, acrylic acid, methacrylic acid, N-isopropylacrylamide (NIPAM), alginate, or polyethylene glycol (PEG), to obtain a sperm cells-embedded gel. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US11644455B2

公开(公告)日：2023-05-09

申请号：US17643058

申请日：2021-12-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: G01N33/483 ,  C12N5/076 ,  G01N21/64

CPC classification number: G01N33/4833 ,  C12N5/061 ,  G01N2021/6439

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US20220196630A1

公开(公告)日：2022-06-23

申请号：US17643058

申请日：2021-12-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: G01N33/483 ,  C12N5/076

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US12153039B2

公开(公告)日：2024-11-26

申请号：US18300322

申请日：2023-04-13

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: G01N33/483 ,  C12N5/076 ,  G01N21/64

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US20230251241A1

公开(公告)日：2023-08-10

申请号：US18300322

申请日：2023-04-13

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: G01N33/483 ,  C12N5/076

CPC classification number: G01N33/4833 ,  C12N5/061 ,  G01N2021/6439

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.