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    Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers
    1.
    发明授权
    Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers 有权
    使用编码蛋白结合RNA适体的RNA相关过程的转录后调控

    公开(公告)号：US09125931B2

    公开(公告)日：2015-09-08

    申请号：US12755121

    申请日：2010-04-06

    申请人： Brian J. Belmont ,  Stephen J. Goldfless ,  Michael A. Marletta ,  Jacquin C. Niles

    发明人： Brian J. Belmont ,  Stephen J. Goldfless ,  Michael A. Marletta ,  Jacquin C. Niles

    IPC分类号： C07H21/04 ,  C12N15/63 ,  A61K31/713 ,  C12N15/115 ,  A61K31/70

    CPC分类号： A61K31/713 ,  C12N15/115 ,  C12N2310/16

    摘要： In vitro SELEX has been used to discover high affinity RNA aptamers interacting with the tetracycline repressor protein in a tetracycline-dependent manner. Using in silico RNA folding predictions to guide the design of both aptamer truncations and mutants, minimized tetracycline repressor protein high affinity binding aptamers have been defined. Using one such aptamer, inducible post-transcriptional regulation in vivo has been demonstrated that is predicated on a direct interaction between a tetracycline repressor protein and a RNA aptamer element. These aptamer components can be integrated in any organism to inducibly regulate RNA translation of a gene of interest.

    摘要翻译： 已经使用体外SELEX发现以四环素依赖的方式与四环素阻遏蛋白相互作用的高亲和力RNA适体。 使用电脑RNA折叠预测来指导适体截短和突变体的设计，最小化四环素阻遏蛋白高亲和力结合适配体已被定义。 已经证明使用一种这样的适体，体内可诱导的转录后调控是以四环素阻遏物蛋白和RNA适体元件之间的直接相互作用为前提的。 这些适配子组分可以整合到任何生物体中以诱导调节目的基因的RNA翻译。

    Post-Transcriptional Regulation Of RNA-Related Processes Using Encoded Protein-Binding RNA Aptamers
    2.
    发明申请
    Post-Transcriptional Regulation Of RNA-Related Processes Using Encoded Protein-Binding RNA Aptamers 有权
    使用编码蛋白结合RNA的RNA相关过程的转录后调控

    公开(公告)号：US20110245326A1

    公开(公告)日：2011-10-06

    申请号：US12755121

    申请日：2010-04-06

    申请人： Brian J. Belmont ,  Stephen J. Goldfless ,  Michael A. Marletta ,  Jacquin C. Niles

    发明人： Brian J. Belmont ,  Stephen J. Goldfless ,  Michael A. Marletta ,  Jacquin C. Niles

    IPC分类号： A61K31/713 ,  C07H21/02 ,  C07K14/00 ,  C12P21/00 ,  A61P43/00 ,  B65D71/00

    CPC分类号： A61K31/713 ,  C12N15/115 ,  C12N2310/16

    摘要： In vitro SELEX has been used to discover high affinity RNA aptamers interacting with the tetracycline repressor protein in a tetracycline-dependent manner. Using in silico RNA folding predictions to guide the design of both aptamer truncations and mutants, minimized tetracycline repressor protein high affinity binding aptamers have been defined. Using one such aptamer, inducible post-transcriptional regulation in vivo has been demonstrated that is predicated on a direct interaction between a tetracycline repressor protein and a RNA aptamer element. These aptamer components can be integrated in any organism to inducibly regulate RNA translation of a gene of interest.

    摘要翻译： 已经使用体外SELEX发现以四环素依赖的方式与四环素阻遏蛋白相互作用的高亲和力RNA适体。 使用电脑RNA折叠预测来指导适体截短和突变体的设计，最小化四环素阻遏蛋白高亲和力结合适配体已被定义。 已经证明使用一种这样的适体，体内可诱导的转录后调控是以四环素阻遏物蛋白和RNA适体元件之间的直接相互作用为前提的。 这些适配子组分可以整合到任何生物体中以诱导调节目的基因的RNA翻译。