公开(公告)号：US06902914B2

公开(公告)日：2005-06-07

申请号：US10002292

申请日：2001-11-15

申请人： Brian Ward ,  Leanne M. Snyder ,  Chuan Li ,  Keming Song ,  Kristen E. Opper ,  Stephanie Uder ,  Ron Hernan

发明人： Brian Ward ,  Leanne M. Snyder ,  Chuan Li ,  Keming Song ,  Kristen E. Opper ,  Stephanie Uder ,  Ron Hernan

IPC分类号： C12N15/66 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12N15/66 ,  C12Q1/6846 ,  C12Q2525/125 ,  C12Q2521/319

摘要： Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.

摘要翻译： 提供扩增混合物，试剂盒，扩增子，试剂盒和方法用于扩增核酸。 特别地，提供了利用包含聚合酶，含有修饰的dNTP的脱氧核苷三磷酸（dNTP）混合物，第一引物和第二引物的扩增混合物的方法。 还提供了dNTP混合物，其含有用于四种核苷酸三磷酸中的至少两种的修饰的dNTP，当掺入多聚苷中时，其在掺入修饰的dNTP的位点赋予外切核酸酶对酶降解的抗性。 还提供了掺入修饰的核苷酸的扩增子和载体。