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    MICROARRAY SYSTEM WITH IMPROVED SEQUENCE SPECIFICITY
    4.
    发明申请
    MICROARRAY SYSTEM WITH IMPROVED SEQUENCE SPECIFICITY 审中-公开

    公开(公告)号：US20170369936A1

    公开(公告)日：2017-12-28

    申请号：US15425882

    申请日：2017-02-06

    申请人： Kerry B. Gunning ,  Mark Aaron Behlke

    发明人： Kerry B. Gunning ,  Mark Aaron Behlke

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  B01J19/00

    CPC分类号： C12Q1/6837 ,  B01J19/0046 ,  B01J2219/0059 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00722 ,  C12P19/34 ,  C12Q1/6806 ,  Y10T436/108331 ,  C12Q2525/125 ,  C12Q2533/101 ,  C12Q2521/319

    摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.

    DETECTION OF NUCLEIC ACIDS BY STRAND INVASION BASED AMPLIFICATION
    6.
    发明申请
    DETECTION OF NUCLEIC ACIDS BY STRAND INVASION BASED AMPLIFICATION 审中-公开
    通过基于入侵的扩增检测核酸

    公开(公告)号：US20160289747A1

    公开(公告)日：2016-10-06

    申请号：US15038439

    申请日：2014-11-21

    申请人： ORION DIAGNOSTICA OY

    发明人： Kevin Eboigbodin ,  Mirko Brummer

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q1/6818 ,  C12Q1/6844 ,  C12Q2600/16 ,  C12Q2521/507 ,  C12Q2525/113 ,  C12Q2537/1373 ,  C12Q2561/113 ,  C12Q2565/1015 ,  C12Q2525/107 ,  C12Q2525/125

    摘要： A method for detecting a target nucleic acid sequence in a sample in the presence of at least protein capable of binding to single-stranded DNA is provided, comprising contacting said sample with at least one oligonucleotide probe comprising a fluorophore, a quencher and a region complementary to said target nucleic acid sequence. The sequence of the oligonucleotide probe comprises at least 20% RNA nucleotides, modified RNA nucleotides and/or PNA nucleotides.

    摘要翻译： 提供一种用于在存在至少能够结合单链DNA的蛋白质的情况下检测样品中的靶核酸序列的方法，包括使所述样品与至少一种寡核苷酸探针接触，所述寡核苷酸探针包含荧光团，猝灭剂和互补区域 至所述靶核酸序列。 寡核苷酸探针的序列包含至少20％的RNA核苷酸，修饰的RNA核苷酸和/或PNA核苷酸。

    Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
    9.
    发明授权
    Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same 有权
    使用包括3'-5'外切核酸酶抗性猝灭剂结构域的FET标记的寡核苷酸的方法和用于实施它们的组合物

    公开(公告)号：US08822673B2

    公开(公告)日：2014-09-02

    申请号：US12945777

    申请日：2010-11-12

    申请人： Quin Chou ,  Dragan Spasic

    发明人： Quin Chou ,  Dragan Spasic

    IPC分类号： C07H19/04 ,  C07H21/04

    CPC分类号： C12Q1/6825 ,  C12Q1/686 ,  C12Q2565/101 ,  C12Q2525/125

    摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.

    摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本方法中，在具有3'→5'核酸外切酶活性的聚合酶和至少一种包含3'→5'外切核酸酶抗性猝灭结构域的FET标记寡核苷酸探针的存在下进行引物延伸反应。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。