公开(公告)号：US20100144555A1

公开(公告)日：2010-06-10

申请号：US12641194

申请日：2009-12-17

申请人： Bruno TOCQUE ,  Laurent Bracco ,  Florence Edon ,  Fabien Schweighoffer

发明人： Bruno TOCQUE ,  Laurent Bracco ,  Florence Edon ,  Fabien Schweighoffer

IPC分类号： C40B50/14 ,  C40B50/02

CPC分类号： C12N15/1072 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/142 ,  C12Q2539/105 ,  C12Q2565/501 ,  C12Q2537/113 ,  C12Q2521/301

摘要： The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.

摘要翻译： 本发明涉及用于鉴定和/或克隆代表与选择性剪接事件相关的定性差异的核酸区域的方法和/或在两个生理情境之间的位于RNA转录基因组区域中的插入，缺失的方法，其包括衍生自测试的RNA的杂交 来自参考情况的cDNA的情况和/或来自测试情况的​​cDNA的双链杂交和/或来自参考情况的cDNA的双链杂交; 以及鉴定和/或克隆代表定性差异的核酸。 本发明还涉及表示通过上述方法获得的两种生理情况之间的定性差异的核酸组合物或组，以及它们作为探针的用途，用于鉴定感兴趣的基因或分子，或者仍然例如在药物基因组学的方法中，以及 分子相对于其治疗和/或毒性作用。 本发明还涉及使用拼接RNA的调节作为用于预测分子毒性和/或功效的标记物，以及作为药物基因组学中的标记物。