公开(公告)号：US11965001B2

公开(公告)日：2024-04-23

申请号：US16927590

申请日：2020-07-13

Applicant: California Institute of Technology

Inventor： Dan I. Piraner ,  Mikhail Shapiro

IPC: C07K14/255

CPC classification number: C07K14/255 ,  C07K2319/73

Abstract: Provided herein are thermomer monomer, thermomer dimers, thermomer monomeric constructs, thermomer dimeric complexes, and related gene expression cassette vectors, cells, surfaces devices, compositions methods and systems, that provide a thermobioswitch suitable to control location and/or binding of cargo moiety of interest in a temperature regulated manner.

公开(公告)号：US10975420B2

公开(公告)日：2021-04-13

申请号：US15384254

申请日：2016-12-19

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mikhail G. Shapiro ,  Dan I. Piraner ,  Mohamad H. Abedi ,  Brittany Moser ,  Audrey Lee-Gosselin

IPC: C12Q1/68 ,  C12Q1/6825 ,  B01L7/00 ,  C12N15/63 ,  C12N15/10 ,  C12N15/82 ,  G01N33/53 ,  C12N15/00

Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

公开(公告)号：US20210324389A1

公开(公告)日：2021-10-21

申请号：US17230998

申请日：2021-04-15

Applicant: California Institute of Technology

Inventor： Mohamad H. Abedi ,  Mikhail Shapiro ,  Dan I. Piraner ,  Justin Lee

IPC: C12N15/63 ,  C07K14/725

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.