公开(公告)号：US20230272466A1

公开(公告)日：2023-08-31

申请号：US17863183

申请日：2022-07-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jacob T. BARLOW ,  Said R. BOGATYREV

IPC: C12Q1/686 ,  C12Q1/6888

CPC classification number: C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

公开(公告)号：US20170189444A1

公开(公告)日：2017-07-06

申请号：US15399711

申请日：2017-01-05

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Sujit S. DATTA ,  Asher Preska STEINBERG ,  Said R. BOGATYREV

IPC: A61K31/765

CPC classification number: A61K31/765 ,  A61K9/0031 ,  A61K9/0036 ,  A61K31/716 ,  A61K31/719 ,  A61K31/732 ,  A61K31/77 ,  A61K35/747 ,  A61K45/06 ,  A61K2300/00

Abstract: Polymeric composition and related methods and systems for regulating the structure of hydrogels are described. In particular, by varying the physiochemical properties of the polymeric composition, the structure of the hydrogels can be reversibly compressed or decompressed.

公开(公告)号：US20210079447A1

公开(公告)日：2021-03-18

申请号：US16927496

申请日：2020-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jacob T. BARLOW ,  Said R. BOGATYREV

IPC: C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.