公开(公告)号：US20230272466A1

公开(公告)日：2023-08-31

申请号：US17863183

申请日：2022-07-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jacob T. BARLOW ,  Said R. BOGATYREV

IPC: C12Q1/686 ,  C12Q1/6888

CPC classification number: C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

公开(公告)号：US20230183818A1

公开(公告)日：2023-06-15

申请号：US17264734

申请日：2019-08-01

Applicant: California Institute of Technology

Inventor： Rustem F. ISMAGILOV ,  Nathan SCHOEPP ,  Tahmineh KHAZAEI ,  Jacob T. BARLOW

IPC: C12Q1/689 ,  C12Q1/02 ,  C12Q1/04

CPC classification number: C12Q1/689 ,  C12Q1/04 ,  C12Q1/025 ,  C12Q2600/106 ,  C12Q2600/158

Abstract: Provided herein are RNA markers and compositions, methods and systems for the related identification and/or uses in methods for detection of antibiotic susceptibility and resistance in a microorganism, and in particular in N. gonorrhoeae.

公开(公告)号：US20210079447A1

公开(公告)日：2021-03-18

申请号：US16927496

申请日：2020-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jacob T. BARLOW ,  Said R. BOGATYREV

IPC: C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.