公开(公告)号：US20170152336A9

公开(公告)日：2017-06-01

申请号：US14785334

申请日：2014-04-17

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： SUBHA RANJAN DAS ,  SAADYAH AVERICK ,  SOURAV K. DEY ,  Krzysztof Matyjaszewski

IPC: C08F251/00

CPC classification number: C08F251/00 ,  C07H1/00 ,  C07H21/00

Abstract: A method of synthesizing a polynucleotide composition includes attaching a compound including at least one initiator or at least one transfer agent for a reversible deactivation radical polymerization to an end of a nucleotide chain assembly immobilized upon a solid phase support during a solid phase synthesis of a polynucleotide so that the initiator or the transfer agent is attacked to the end of a nucleotide chain assembly in a manner which is stable under conditions of deprotection of the polynucleotide, and growing a polymer from the initiator of from a site of the chain transfer agent via the reversible deactivation radical polymerization to form the polynucleotide composition.

公开(公告)号：US20160075810A1

公开(公告)日：2016-03-17

申请号：US14785334

申请日：2014-04-17

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： SUBHA RANJAN DAS ,  SAADYAH AVERICK ,  SOURAV K. DEY ,  Krzysztof Matyjaszewski

IPC: C08F251/00

CPC classification number: C08F251/00 ,  C07H1/00 ,  C07H21/00

Abstract: A method of synthesizing a polynucleotide composition includes attaching a compound including at least one initiator or at least one transfer agent for a reversible deactivation radical polymerization to an end of a nucleotide chain assembly immobilized upon a solid phase support during a solid phase synthesis of a polynucleotide so that the initiator or the transfer agent is attacked to the end of a nucleotide chain assembly in a manner which is stable under conditions of deprotection of the polynucleotide, and growing a polymer from the initiator of from a site of the chain transfer agent via the reversible deactivation radical polymerization to form the polynucleotide composition.

Abstract translation: 合成多核苷酸组合物的方法包括将固定在固相载体上的核苷酸链组装的末端固定在多核苷酸的固相合成期间将包含至少一种引发剂或至少一种用于可逆失活自由基聚合的转移剂的化合物 使得引发剂或转移剂以在多核苷酸去保护的条件下稳定的方式侵袭到核苷酸链组装的末端，并且通过经由链转移剂的位点从引发剂生长聚合物 可逆失活自由基聚合以形成多核苷酸组合物。