公开(公告)号：US20230416786A1

公开(公告)日：2023-12-28

申请号：US18253977

申请日：2021-11-30

Applicant: CELLECTIS SA

Inventor： Alexandre JUILLERAT ,  Philippe DUCHATEAU ,  Ming YANG

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/86 ,  C07D215/46

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/86 ,  C07D215/46 ,  C12N2750/14143

Abstract: The invention provides aminoquinoline compounds as powerful enhancers of genetic recombination in living cells, especially to perform site-directed gene integration of exogenous DNA template by homologous recombination. In particular, disclosed are methods by which cells are treated with chloroquine and/or hydroxychloroquine prior to, or concomitantly with, the introduction of exogenous DNA templates, and optionally in presence of rare-cutting endonucleases, to obtain higher rates of gene integration or correction.