公开(公告)号：US20140056814A1

公开(公告)日：2014-02-27

申请号：US13945880

申请日：2013-07-18

Applicant: CELLSEED INC.

Inventor： Teruo OKANO ,  Akihiko KIKUCHI ,  Masayuki YAMATO ,  Akira MASUDA

IPC: A01K67/027 ,  C12N5/09

CPC classification number: A01K67/0271 ,  A01K2227/105 ,  A01K2267/0331 ,  A61K49/0008 ,  C12N5/0693

Abstract: A cell culture support is first prepared that is coated on a surface with a polymer the hydration force of which changes in a temperature range of 0-80° C.; cancer cells are then cultivated on the support in a temperature region where the polymer has weak hydration force; thereafter, the culture solution is adjusted to a temperature at which the polymer has a stronger hydration force, whereby the cultured cancer cells are detached; the detached cancer cells are then transplanted to a specified site of an animal on which transplantation is to be performed; this method is an efficient way of cancer cells transplantation.

Abstract translation: 首先制备细胞培养载体，其在表面上涂覆聚合物，其水合力在0-80℃的温度范围内变化; 然后将癌细胞在聚合物具有弱水合力的温度区域中在载体上培养; 此后，将培养液调整为聚合物具有更强的水合力的温度，由此分离培养的癌细胞。 然后将分离的癌细胞移植到要进行移植的动物的特定部位; 这种方法是癌细胞移植的有效途径。