公开(公告)号：US20140212470A1

公开(公告)日：2014-07-31

申请号：US14228212

申请日：2014-03-27

Applicant: CELLSEED INC.

Inventor： Masato KANZAKI ,  Masayuki YAMATO ,  Tinatsu KOHNO ,  Hidekazu SEKINE ,  Akihiko KIKUCHI ,  Teruo OKANO ,  Takamasa ONUKI

IPC: A61L27/38 ,  C12N5/071

CPC classification number: A61L27/3895 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  C12N5/0068 ,  C12N5/0688 ,  C12N2533/30

Abstract: The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.

Abstract translation: 本发明要解决的问题是提供具有优异组织粘附性和优异柔韧性的培养细胞片。 可以通过在表面活性蛋白或交联抑制剂的存在下培养细胞培养的细胞片来解决问题，所述细胞片在涂覆有具有上或下的温度响应性聚合物的细胞培养载体的表面上 包括以下步骤：将培养基的温度调节到低于临界溶解温度以下或高于临界溶解温度以上的温度; 并分离培养的细胞片。

公开(公告)号：US20140056814A1

公开(公告)日：2014-02-27

申请号：US13945880

申请日：2013-07-18

Applicant: CELLSEED INC.

Inventor： Teruo OKANO ,  Akihiko KIKUCHI ,  Masayuki YAMATO ,  Akira MASUDA

IPC: A01K67/027 ,  C12N5/09

CPC classification number: A01K67/0271 ,  A01K2227/105 ,  A01K2267/0331 ,  A61K49/0008 ,  C12N5/0693

Abstract: A cell culture support is first prepared that is coated on a surface with a polymer the hydration force of which changes in a temperature range of 0-80° C.; cancer cells are then cultivated on the support in a temperature region where the polymer has weak hydration force; thereafter, the culture solution is adjusted to a temperature at which the polymer has a stronger hydration force, whereby the cultured cancer cells are detached; the detached cancer cells are then transplanted to a specified site of an animal on which transplantation is to be performed; this method is an efficient way of cancer cells transplantation.

Abstract translation: 首先制备细胞培养载体，其在表面上涂覆聚合物，其水合力在0-80℃的温度范围内变化; 然后将癌细胞在聚合物具有弱水合力的温度区域中在载体上培养; 此后，将培养液调整为聚合物具有更强的水合力的温度，由此分离培养的癌细胞。 然后将分离的癌细胞移植到要进行移植的动物的特定部位; 这种方法是癌细胞移植的有效途径。

公开(公告)号：US20170067861A1

公开(公告)日：2017-03-09

申请号：US15354108

申请日：2016-11-17

Applicant: Hideko KANAZAWA ,  CELLSEED INC. ,  TOKYO WOMEN'S MEDICAL UNIVERSITY

Inventor： Kenichi NAGASE ,  Jun KOBAYASHI ,  Akihiko KIKUCHI ,  Yoshikatsu AKIYAMA ,  Hideko KANAZAWA ,  Teruo OKANO

IPC: G01N30/54 ,  B01J20/28 ,  B01J20/283 ,  C07J1/00 ,  B01J20/32 ,  B01D15/38 ,  B01D15/22 ,  C07J7/00 ,  B01J20/26 ,  B01J20/288

CPC classification number: G01N30/54 ,  B01D15/22 ,  B01D15/3876 ,  B01D15/42 ,  B01J20/267 ,  B01J20/28042 ,  B01J20/283 ,  B01J20/288 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/327 ,  B01J2220/825 ,  C07J1/00 ,  C07J7/009 ,  G01N30/50 ,  G01N2030/528

Abstract: A temperature responsive monolithic porous material is obtained that comprises a polymer having a hydration ability that changes in a temperature range of 0 to 80° C. and being immobilized to a surface of the porous material at a high density by binding an atom transfer radical polymerization initiator to a surface of the porous material, and inducing a growth reaction of a polymer, having a hydration ability that changes in a temperature range of 0 to 80° C., from the initiator using an atom transfer radical process under a presence of a catalyst.

Abstract translation: 得到一种温度响应整体多孔材料，其包含具有在0至80℃的温度范围内变化的水合能力的聚合物，并通过结合原子转移自由基聚合以高密度固定在多孔材料的表面上 引发剂到多孔材料的表面，并且使用原子转移自由基法在具有0至80℃的温度范围内变化的水合能力的聚合物的生长反应下，在 催化剂。