公开(公告)号：US20110157692A1

公开(公告)日：2011-06-30

申请号：US12851535

申请日：2010-08-05

申请人： CHE-HSIN LIN ,  Shi-wei Lin ,  Jui-hung Hsu ,  Chih-han Chang

发明人： CHE-HSIN LIN ,  Shi-wei Lin ,  Jui-hung Hsu ,  Chih-han Chang

IPC分类号： G02B21/06

CPC分类号： G02B21/10 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/474 ,  G01N21/645 ,  G01N2021/4704 ,  G01N2021/6421

摘要： An objective-type dark-field illumination device for a microfluidic channel is provided and includes an optical stop having a pair of symmetric curved slits used to adjust the optical path and inner numerical aperture of a dark-field light source generated by the device. The dark-field illumination can focus on a smaller spot to illuminate a sample in the microfluidic channel by matching a pin-hole combined with a transmitter objective lens. The optical path and smaller spot is advantageous to solve the problem of a traditional dark-field illumination that may generate background light noise scattered from inner walls of the microfluidic channel to lower the image contrast. Therefore, the signal or image resolution of capturing the scattered light and/or emitted fluorescent light emitted from the sample in the microfluidic channel can be enhanced. Meanwhile, the device can simultaneously excite and detect multiple fluorescent samples with different excited wavelengths in the microfluidic channel.

摘要翻译： 提供了一种用于微流体通道的目标型暗视场照明装置，包括具有一对对称弯曲狭缝的光学停止件，用于调节由该装置产生的暗视场光源的光程和内数值孔径。 通过匹配与发射器物镜组合的针孔，暗场照明可以聚焦在较小的点上以照射微流体通道中的样品。 光路和较小的斑点有利于解决可能产生从微流体通道的内壁散射的背景光噪声以降低图像对比度的传统暗场照明的问题。 因此，可以提高捕获从微流体通道中的样品发射的散射光和/或发射的荧光的信号或图像分辨率。 同时，器件可以在微流体通道中同时激发和检测具有不同激发波长的多个荧光样品。