公开(公告)号：US11110371B2

公开(公告)日：2021-09-07

申请号：US16489510

申请日：2017-12-27

Applicant: CHIYODA CORPORATION ,  CONFOCAL SCIENCE INC.

Inventor： Naoki Tanigawa ,  Hiroaki Tanaka ,  Sachiko Takahashi ,  Koji Inaka

IPC: C30B29/58 ,  B01D9/00 ,  B01D61/36 ,  C12N9/36

Abstract: Provided are: an economically superior protein crystallization method capable of efficiently finding conditions for crystallization by using a small amount of protein; and a crystallization device used for the method. According to the present invention, a transparent sealed container 1 is filled with a solution of protein, a part of the transparent sealed container 1 being formed of a semipermeable membrane 2 with a molecular weight cut-off that inhibits passage of the protein while allowing passage of a precipitant, and then, a precipitant solution with changed concentration and/or pH of the precipitant is continuously supplied to the semipermeable membrane 2, to crystallize the protein with the precipitant that infiltrates from the semipermeable membrane 2 into the sealed container 1.