公开(公告)号：US20150147775A1

公开(公告)日：2015-05-28

申请号：US14547487

申请日：2014-11-19

Applicant: CORNING INCORPORATED

Inventor： Derek Michael Fiacco ,  Jeffrey Glenn Lynn ,  Kaitlyn Mary Matias ,  Florence Christine Monique Verrier ,  Ying Wei

IPC: C12Q1/20 ,  C12N1/20

CPC classification number: C12Q1/20 ,  C12N1/20 ,  G01N2333/21 ,  G01N2333/245 ,  G01N2333/265 ,  G01N2333/31

Abstract: A method that includes the steps: inoculating nutrient agar with bacterial stock to form a culture; incubating the culture to form a first incubated culture; incubating a portion of the first culture with nutrient agar to form a second culture; incubating a portion of the second culture to form a third culture; incubating the third culture to form an inoculated test plate; forming an inoculum by suspending bacteria from the inoculated test plate in a buffered test solution, adjusting the pH to ˜7 to 8, and adding organic soil at a concentration of approximately 10% to 30% by weight; inoculating a silver-containing surface region of a test carrier with a portion of the inoculum; incubating the inoculated test carrier; washing the test carrier in a neutralizing solution to form a residual test inoculum; and calculating the percent reduction in the number of surviving bacterial colonies in the residual test inoculum.

Abstract translation: 一种包括以下步骤的方法：用细菌储存培养营养琼脂以形成培养物; 孵育培养物以形成第一培养培养物; 将一部分第一培养物与营养琼脂孵育以形成第二培养物; 孵育一部分第二培养物以形成第三培养物; 培养第三培养物以形成接种的测试板; 通过将接种的测试板上的细菌悬浮在缓冲的测试溶液中，将pH调节至约7至8并加入浓度为约10重量％至30重量％的有机土壤来形成接种物; 用一部分接种物接种试验载体的含银表面区域; 培养接种的试验载体; 在中和溶液中洗涤测试载体以形成残留测试接种物; 并计算残留测试接种物中存活的细菌菌落数量的百分比减少。