公开(公告)号：US20230313232A1

公开(公告)日：2023-10-05

申请号：US18175790

申请日：2023-02-28

Applicant: CORNING INCORPORATED

Inventor： Carine Cerato-Noyerie ,  Samantha Sophie Catherine Haller ,  Davis Henry ,  Marylène Denise Madeleine Pécheul ,  Corinne Walerack

IPC: C12N15/87 ,  C12M1/00

CPC classification number: C12N15/87 ,  C12M29/10

Abstract: Methods and systems are provided for transfecting cells using real-time, continuous transfection of cells. In some aspects, the methods can be applied for the continuous production of non-viral vector nucleic acid complexes. The systems and methods include a passive mixing fluidic module with at least two inlets, a plurality of mixing elements, and an outlet to provide a continuous flow of transfection complexes to a cell reactor. The transfection agent and nucleic acid are passively mixed and then provided to cells in a continuous flow of cell medium. In some aspects, the flow of cell medium perfusing through the cell reactor recirculates. The system and the methods of the present disclosure provide for highly reproducible and scalable transfection with a low coefficient of variation.

公开(公告)号：US20200291348A1

公开(公告)日：2020-09-17

申请号：US16765722

申请日：2018-11-20

Applicant: CORNING INCORPORATED

Inventor： Marie-Maud Bear ,  David Henry ,  Martial Hervy ,  Marylène Denise Madeleine Pécheul ,  Corinne Walerack

IPC: C12N5/00

Abstract: A dissolvable foam scaffold for cell culture is provided herein. The dissolvable foam scaffold includes an ionotropically crosslinked polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid, partially amidated pectic acid and salts thereof, and at least one first water-soluble polymer having surface activity.