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公开(公告)号：US3770589A
公开(公告)日：1973-11-06
申请号：US3770589D
申请日：1971-05-20
CPC分类号： C12N9/92 , Y10S435/814 , Y10S435/822 , Y10S435/832 , Y10S435/837 , Y10S435/84 , Y10S435/848 , Y10S435/853 , Y10S435/863 , Y10S435/866 , Y10S435/886 , Y10S435/906 , Y10S435/921
摘要： An improved process for the production of glucose isomerase involves the addition of a specific amount of glycine (aminoacetic acid) to the fermentation medium. This increases the amount of the enzyme, glucose isomerase, that is produced by the microorganism, which preferably is a strain of Streptomyces, most preferably, S. olivochromogenes ATCC 21,114. Even further improvement is obtained by also adding a small amount of ammonium nitrate to the culture medium.
摘要翻译： 用于生产葡萄糖异构酶的改进方法包括向发酵培养基中加入特定量的甘氨酸（氨基乙酸）。 这增加了由微生物产生的酶，葡萄糖异构酶的量，其优选是链霉菌菌株，最优选S.olivochromogenes ATCC 21,114。 通过向培养基中加入少量的硝酸铵也能进一步改善。
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2.发明授权
公开(公告)号：US3847741A
公开(公告)日：1974-11-12
申请号：US29439772
申请日：1972-10-02
CPC分类号： C12N11/14 , C12N9/92 , C12P19/24 , C13K11/00 , Y10S435/813 , Y10S435/886
摘要： Levulose-bearing syrups are produced by passing a dextrose solution through a column or bed of an immobilized dextrose isomerase enzyme preparation. The useful life of the enzyme preparation is extended by conducting the isomerization initially at a low operating temperature, then increasing the temperature of operation after an initial decrease in enzyme activity has been observed.
摘要翻译： 通过将葡萄糖溶液通过固定化的葡萄糖异构酶制剂的柱或床来产生含有左旋糖的糖浆。 酶制剂的使用寿命通过最初在低操作温度下进行异构化来延长，然后观察到在酶活性初始降低后增加操作温度。
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公开(公告)号：US3847740A
公开(公告)日：1974-11-12
申请号：US40175773
申请日：1973-09-28
CPC分类号： C12N11/14 , C12P19/24 , Y10S435/886
摘要： Levulose-bearing syrups are produced by passing a dextrose solution through a column or bed of an immobilized dextrose isomerase enzyme preparation. The useful life of the enzyme preparation is extended by conducting the isomerization initially at a low operating temperature, then increasing the temperature of operation after an initial decrease in enzyme activity has been observed. Also disclosed is an improved immobilized xylose isomerase enzyme preparation which is prepared by contacting a solution of xylose isomerase with particulate basic magnesium carbonate. The novel immobilized enzyme preparation is useful in enzymatically isomerizing dextrose solutions to levulose-bearing syrups.
摘要翻译： 通过将葡萄糖溶液通过固定化的葡萄糖异构酶制剂的柱或床来产生含有左旋糖的糖浆。 酶制剂的使用寿命通过最初在低操作温度下进行异构化来延长，然后观察到在酶活性初始降低之后增加操作温度。 还公开了通过使木糖异构酶与颗粒状碱式碳酸镁接触来制备的改进的固定化木糖异构酶制剂。 新型固定化酶制剂可用于将含葡萄糖的糖浆的葡萄糖溶液酶促异构化。
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公开(公告)号：US3813318A
公开(公告)日：1974-05-28
申请号：US18163971
申请日：1971-09-17
发明人： ARMBRUSTER F , HEADY R , CORY R
CPC分类号： C12N9/92 , C12P19/24 , Y10S435/886
摘要： PROCESS FOR THE PRODUCTION OF XYLOSE (DEXTROSE) ISOMERASE BY MEANS OF A MUTANT STRAIN OF STREPTOMYCES THAT PROLEFERATES IN A CULTURE MEDIUM THAT MAY BE FREE OF XYLOSE. THE XYLOSE (DEXTROSE) ISOMERASE ENZYMES IS THEREFORE CONSTITUTIVE RATHER THAN INDUCED.
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公开(公告)号：US3806419A
公开(公告)日：1974-04-23
申请号：US23444072
申请日：1972-03-10
发明人： HEADY R
CPC分类号： C12Y302/01041 , C12N9/2457 , Y10S435/813 , Y10S435/822
摘要： A METHOD FOR MAKING A PULLULANASE ENZYME PREPARATION. THE METHOD INVOLVES MAKING A PULLULANASE ENZYME PREPARATION FROM THE AEROBACTER AEROGENES BACTERIUM BY PROVIDING AS A CARBOHYDRATE SOURCE EITHER A STARCH OR A LOW D.E STARCH HYDROLYSATE IN EITHER THE CULTURE MAINTENANCE STEP OR THE INOCULUM DEVELOPMENT STAGE.
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