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    Escherichia coli strain for an oral vaccine against post-weaning diarrhea in pigs
    2.
    发明授权
    Escherichia coli strain for an oral vaccine against post-weaning diarrhea in pigs 失效
    用于针对猪断奶后腹泻的口服疫苗的大肠杆菌菌株

    公开(公告)号：US07163820B1

    公开(公告)日：2007-01-16

    申请号：US09958081

    申请日：2000-03-29

    Applicant: Bela Nagy ,  Ferenc Olasz ,  Zsolt Fekete

    Inventor： Bela Nagy ,  Ferenc Olasz ,  Zsolt Fekete

    IPC: C12N1/00 ,  C12N15/00 ,  C12N1/20 ,  C12N15/63 ,  C12N15/09 ,  A01N63/00 ,  A61K48/00

    CPC classification number: C12R1/19 ,  C07K14/245 ,  Y10S435/848 ,  Y10S435/849

    Abstract: The invention relates to a strain suitable for producing a live, orally applicable Escherichia coli vaccine for the prevention of post-weaning diarrhoea in pigs, and the procedure suitable for producing that strain. The essence of the strain is that the enterotoxin-free and originally wild-type Escherichia coli strain simultaneously produces two adhesive fimbriae (F4 and F18), whereas the essence of the procedure is that the enterotoxin-producing ability if the wild, pathogenic, enterotoxigenic Escherichia coli strain originally capable of producing enterotoxins and F18 fimbriae is abolished by a genetic intervention while retaining the ability of the strain to produce F18 fimbria facilitating adhesion to the small intestinal wall of weaned piglets, and subsequently the strain thus modified is rendered capable of producing a further surface adhesion fimbria (F4).

    Abstract translation: 本发明涉及适用于生产用于预防猪的断奶后腹泻的活的，口服适用的大肠杆菌疫苗的菌株，以及适于产生该菌株的方法。 该菌株的本质是无肠毒素和最初野生型大肠杆菌菌株同时产生两种粘性菌毛（F4和F18），而程序的本质是产生肠毒素的能力，如果野生，致病性，肠毒素 最初能够产生肠毒素和F18菌毛的大肠杆菌菌株通过遗传干预而被消除，同时保留菌株产生F18菌毛的能力，促进对断奶仔猪的小肠壁的粘附，随后由此改变的菌株能够产生 进一步的表面粘附fimbria（F4）。

    Enzymatic method for detecting coliform bacteria or E. coli
    8.
    发明授权
    Enzymatic method for detecting coliform bacteria or E. coli 失效
    用于检测大肠杆菌或大肠杆菌的酶法

    公开(公告)号：US5861270A

    公开(公告)日：1999-01-19

    申请号：US836140

    申请日：1997-07-14

    Applicant: Jozef Constantia Frans Hans Nelis

    Inventor： Jozef Constantia Frans Hans Nelis

    IPC: C12Q1/04 ,  C12N1/20 ,  C12Q1/10 ,  C12Q1/40 ,  C12R1/19 ,  C12Q1/54 ,  G01N33/53

    CPC classification number: C12N1/20 ,  C12Q1/10 ,  G01N2333/245 ,  Y10S435/848 ,  Y10S435/849 ,  Y10S435/968

    Abstract: A two stage enzymatic method for the detection of coliform bacteria or E. coli wherein bacteria are concentrated on a membrane filter. This filter is placed on a growth medium containing nutrients, including preferably minerals, a protein hydrolysate and a sugar, preferably maltose or a polyalcohol, preferably mannitol, an inducer of a marker enzyme, in particular .beta.-galactosidase or .beta.-glucuronidase and inhibitors of the growth of competing bacteria. After a preincubation step, the filter is placed on an assay medium containing a fluorogenic or chemiluminogenic enzyme substrate and a membrane permeabilizer. The membrane filter and the assay medium are incubated to allow cleavage of the enzyme substrate producing fluorescent or chemiluminescent microcolonies on the membrane filter after triggering of light emission.

    Abstract translation: PCT No.PCT / BE95 / 00102 Sec。 371日期1997年7月14日 102（e）日期1997年7月14日PCT提交1995年11月7日PCT公布。 公开号WO96 / 14431 日期1996年5月17日用于检测大肠菌群或大肠杆菌的两阶段酶法，其中细菌浓缩在膜过滤器上。 该过滤器放置在含有营养物质的生长培养基上，其中优选矿物质，蛋白质水解物和糖，优选麦芽糖或多元醇，优选甘露醇，标记酶的诱导物，特别是β-半乳糖苷酶或β-葡糖醛酸糖苷酶，以及抑制剂 竞争性细菌的生长。 在预温育步骤之后，将过滤器放置在含有荧光或化学发光酶底物和膜渗透剂的测定培养基上。 孵育膜过滤器和测定培养基以允许在触发发光之后，在膜滤器上产生荧光或化学发光微菌落的酶底物的切割。

    Oligonucleotides for detecting bacteria and detection method using same
    10.
    发明授权
    Oligonucleotides for detecting bacteria and detection method using same 失效
    用于检测细菌的寡核苷酸及其检测方法

    公开(公告)号：US5525718A

    公开(公告)日：1996-06-11

    申请号：US379296

    申请日：1995-01-27

    Applicant: Tetsuo Ohashi ,  Jun Tada ,  Shigeru Fukushima ,  Hiroko Ozaki ,  Naoyuki Nishimura ,  Yoshinari Shirasaki ,  Koichi Yamagata

    Inventor： Tetsuo Ohashi ,  Jun Tada ,  Shigeru Fukushima ,  Hiroko Ozaki ,  Naoyuki Nishimura ,  Yoshinari Shirasaki ,  Koichi Yamagata

    IPC: C12Q1/68 ,  C07H21/04 ,  C12N1/00 ,  C12N15/00

    CPC classification number: C12Q1/689 ,  Y10S435/848 ,  Y10S435/909

    Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

    Abstract translation: 可以与副溶血弧菌的特异性基因选择性杂交的寡核苷酸（SEQ ID NO：1-8），可选择性地与产生性埃希氏弧线圈的LT基因杂交的寡核苷酸（SEQ ID NO：9-13），选择性可混合的寡核苷酸（SEQ ID NO：14-21） 与产毒性埃希氏胆碱圈的STh或STp基因，选择性地与金黄色葡萄球菌的entA，B，C或D基因杂交的寡核苷酸（SEQ ID NO：22-47）或选择性地与金黄色葡萄球菌选择性杂交的寡核苷酸（SEQ ID NO：48-53） 制备Staplyloccus金黄色葡萄球菌的entE基因并用作基因扩增的引物，从而仅选择性地检测引起食物中毒的相应微生物。

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