公开(公告)号：US20160319368A1

公开(公告)日：2016-11-03

申请号：US15105029

申请日：2014-12-17

申请人： CSIR

发明人： Jerolen Naidoo ,  Samantha Barichievy ,  Rethabile Khutlang ,  Musa M. Mhlanga

IPC分类号： C12Q1/68 ,  A61K31/4439 ,  A61K31/58 ,  A61K31/197 ,  A61K31/454 ,  A61K31/506 ,  A61K31/496 ,  A61K33/24 ,  A61K31/4709 ,  A61K38/12 ,  A61K31/517 ,  A61K31/7072 ,  A61K31/5377 ,  A61K31/501 ,  A61K31/675 ,  A61K31/519 ,  C01G55/00 ,  C12N15/113

CPC分类号： C12Q1/6886 ,  A61K31/197 ,  A61K31/436 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/454 ,  A61K31/4709 ,  A61K31/475 ,  A61K31/495 ,  A61K31/496 ,  A61K31/501 ,  A61K31/5025 ,  A61K31/506 ,  A61K31/517 ,  A61K31/519 ,  A61K31/5377 ,  A61K31/58 ,  A61K31/675 ,  A61K31/7072 ,  A61K31/713 ,  A61K33/24 ,  A61K38/12 ,  C12N15/111 ,  C12N15/1132 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/30 ,  C12Q1/703 ,  C12Q2600/136 ,  C12Q2600/178

摘要： The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.

摘要翻译： 本发明涉及使用高通量筛选方法鉴定抗HIV miRNA和抗HIV药物化合物的方法，其包括：用一组miRNA转染报道细胞，用HIV感染报道细胞，筛选细胞以鉴定 调节HIV感染并鉴定由miRNA靶向的特异性途径，核酸和/或多肽的miRNA。 本发明进一步提供了鉴定和筛选具有针对由miRNA靶向用于治疗HIV的功效的特异性途径，核酸和/或多肽的已知活性的抗HIV药物化合物。 本发明还提供了miRNA模拟物，miRNA抑制剂和药物化合物（包括肿瘤学药物和激酶抑制剂）在治疗和/或预防HIV感染中的应用。

公开(公告)号：US10214779B2

公开(公告)日：2019-02-26

申请号：US15694084

申请日：2017-09-01

申请人： CSIR

发明人： Jerolen Naidoo ,  Samantha Barichievy ,  Rethabile Khutlang ,  Musa M Mhlanga

IPC分类号： C12Q1/6886 ,  C12N15/113 ,  A61K38/12 ,  A61K31/436 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/454 ,  A61K31/475 ,  A61K31/495 ,  A61K31/496 ,  A61K31/501 ,  A61K31/5025 ,  A61K31/506 ,  A61K31/517 ,  A61K31/519 ,  A61K31/5377 ,  A61K31/675 ,  A61K31/713 ,  A61K33/24 ,  C12Q1/70 ,  A61K31/197 ,  A61K31/4709 ,  A61K31/58 ,  A61K31/7072 ,  C12N15/11

摘要： The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.

公开(公告)号：US10351916B2

公开(公告)日：2019-07-16

申请号：US15962645

申请日：2018-04-25

申请人： CSIR

发明人： Jerolen Naidoo ,  Samantha Barichievy ,  Rethabile Khutlang ,  Musa M Mhlanga

IPC分类号： A61K31/19 ,  C12Q1/6886 ,  C12N15/113 ,  A61K38/12 ,  A61K31/436 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/454 ,  A61K31/475 ,  A61K31/495 ,  A61K31/496 ,  A61K31/501 ,  A61K31/5025 ,  A61K31/506 ,  A61K31/517 ,  A61K31/519 ,  A61K31/5377 ,  A61K31/675 ,  A61K31/713 ,  A61K33/24 ,  C12Q1/70 ,  A61K31/197 ,  A61K31/4709 ,  A61K31/58 ,  A61K31/7072 ,  C12N15/11

摘要： The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.

公开(公告)号：US10143691B2

公开(公告)日：2018-12-04

申请号：US15503610

申请日：2015-08-17

申请人： CSIR

发明人： Janine Scholefield ,  Jerolen Naidoo ,  Musa M Mhlanga ,  Samantha Barichievy

IPC分类号： A61K31/5025 ,  A61K38/17 ,  A61K31/277 ,  A61K31/497 ,  A61K31/496

摘要： The present invention relates to methods and compositions for modulating the control of apoptosis, specifically for modulating the interaction between hnRNP-K and lincRNA-p21 to induce or prevent apoptosis in the cell.