公开(公告)号：US20240310368A1

公开(公告)日：2024-09-19

申请号：US18400491

申请日：2023-12-29

Applicant: Calibra Scientific, Inc.

Inventor： Pengyun LIU ,  Meihua WANG ,  Yikun LI ,  Weijia WU ,  Huafen LIU

IPC: G01N33/543

CPC classification number: G01N33/54326

Abstract: The present invention provides a detection kit for catecholamines and metabolites thereof in plasma/urine and a detection method and use thereof. By adding a stabilizer into plasma and adding an acid into urine, substances to be tested in plasma/urine samples are maintained stable. The substances to be tested in the samples are adsorbed and extracted by a magnetic medium composite material, and then the contents of the catecholamines and metabolites thereof are detected by high performance liquid chromatography tandem mass spectrometry. The method is simple, efficient and suitable for samples from different sources, and has the advantages of good universality, high stability and long-term storage of samples, a high automation degree of an operation process, short time consumed and high sensitivity. The substances to be tested in the plasma and the urine are simultaneously extracted and detected by the same kit and the same detection method, so as to realize standardized operation for detection of the catecholamines and metabolites thereof.