公开(公告)号：US20220290162A1

公开(公告)日：2022-09-15

申请号：US17692778

申请日：2022-03-11

Applicant: California Institute of Technology

Inventor： Mohamad H. Abedi ,  Mikhail G. Shapiro ,  Michael S. Yao

IPC: C12N15/63 ,  C12N15/90 ,  C12N9/22

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of probiotic cells. There are provided, in some embodiments, thermal bioswitches that allow probiotic cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. Thermally actuated probiotic cells and methods of use for the treatment of diseases or disorders are also provided.