公开(公告)号：US20230167443A1

公开(公告)日：2023-06-01

申请号：US17911184

申请日：2021-03-16

Applicant: Cancer Research Technology Limited

Inventor： Iain Alasdair RUSSELL

IPC: C12N15/113 ,  C12N15/10 ,  C12N9/22

CPC classification number: C12N15/113 ,  C12N15/102 ,  C12N9/22 ,  C12N2310/20 ,  C12N2320/11

Abstract: The invention provides methods of selecting guide RNA sequences, and the use of such sequences in CRISPR-Cas gene editing of a target sequence. In particular, the invention relates to a method of selecting guide RNA sequences, based on the determined frequencies of editing outcomes, which in one case result in low mosaicism and in another case result in large deletions or knockouts.